- Full text PDF available (0)
- This year (0)
- Last 5 years (0)
- Last 10 years (0)
Journals and Conferences
This paper is an attempt to the analysis of the main biochemical characteristics of alkaline phosphatase from sheep polymorphonuclear neutrophils. Ten male adult Romanoff X Berrichon sheep were studied. Alkaline phosphatase was analyzed from cell homogenates, after extraction and solubilization steps. The Vmax and Km values for 4-nitrophenylphosphate at pH… (More)
The kinetics of neutrophil alkaline phosphatase (AP) inactivation by urea were examined. In standardized conditions (molarity and pH of buffer, magnesium chloride and urea concentration), monitoring of the enzymatic reaction allows identification of neutrophil AP isoenzymes. This study confirms the heterogeneity of AP in normal human neutrophils.
In neutrophils from a 75-year-old woman suffering from chronic leukemia, an abnormal alkaline phosphatase (ALP) was observed, characterized by a significant increase in neutrophil alkaline phosphatase (NAP) activity, associated with an ectopic expression of two isoenzymes: a thermolabile ALP similar to the early placental form (or non-Regan variant), and a… (More)