J. D. Glazier

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UNLABELLED System A-mediated amino acid transport across the placenta is important for the supply of neutral amino acids needed for fetal growth. All three system A subtypes (SNAT1, 2, and 4) are expressed in human placental trophoblast suggesting there is an important biological role for each. Placental system A activity increases as pregnancy progresses,(More)
E. E. Champion, S. J. Mann, J. D. Glazier, C. J. P. Jones, J. M. Rawlings, C. P. Sibley, and S. L. Greenwood Academic Unit of Child Health, University of Manchester, St. Mary’s Hospital, Manchester, M13 OJH; Waltham Centre for Pet Nutrition, Waltham on the Wolds, Melton Mowbray, Leicestershire, LE14 4RT; and Academic Unit of Obstetrics and Gynaecology,(More)
The purpose of this study was to compare Na+/H+ exchanger (NHE) activity in the microvillous (MVM) and basal (BM) plasma membrane of the human placental syncytiotrophoblast and to determine the relative contribution of various NHE isoforms to this activity. Uptake of 22Na into isolated MVM vesicles in the presence of a H+ gradient, at initial rate, was(More)
Placental amino acid transport is required for fetal development and impaired transport has been associated with poor fetal growth. It is well known that placental amino acid transport is mediated by a broad array of specific membrane transporters with overlapping substrate specificity. However, it is not fully understood how these transporters function,(More)
We tested the hypothesis that diabetes in pregnancy can result in the in-utero reprogramming of renal calcium and magnesium handling and of bone formation in the offspring, which persists into adulthood. Male offspring of streptozotocin-treated diabetic rats (OD rats) and of control non-diabetic animals (OC rats) were investigated as neonates and at 8, 12(More)
Placental amino acid transfer is essential for fetal development and its impairment is associated with poor fetal growth. Amino acid transfer is mediated by a broad array of specific plasma membrane transporters with overlapping substrate specificity. However, it is not fully understood how these different transporters work together to mediate net flux(More)
The system A amino acid transporter is encoded by three members of the Slc38 gene family giving rise to three subtypes: SNAT1, SNAT2, and SNAT4. SNAT2 is expressed ubiquitously in mammalian tissues, SNAT1 is predominantly expressed in heart, brain, and placenta while SNAT4 is reported to be expressed uniquely by the liver. In the placenta, system A has an(More)
"It is therefore quite futile to inject some substance . . . into the maternal circulation of the rat at the end of pregnancy, and then, if it goes through into the foetal circulation, to report that 'the placenta is permeable to' whatever it was that was injected. Yet such wide conclusions have only too often been drawn although the only right conclusion(More)
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