Learn More
Two distinctly different glutamine synthetase enzymes (EI and EII) have been isolated from the extreme thermophile Bacillus caldolyticus, grown on chemically defined medium at 70 degrees C. Purification to homogeneity mainly involves affinity chromatography and heat treatment with substrate protection. Biosynthesis of total enzyme activity can be repressed(More)
Glutamine synthetase has been purified to homogeneity from B. subtilis (37 degrees) B. stearothermophilus (55 degrees), and B. caldolyticus (75 degrees). Those characteristics compared include size (6.0 +/- 0.3 X 10(5) daltons), quaternary structure (12 SU) amino acid content, substrate Km's and specificity for structural analogs, metal ion activation,(More)
To determine the effect of nutritional status on the medical course and rehabilitation outcome of patients on an adult rehabilitation service, serum albumin (SA) and total lymphocyte count (TLC) were prospectively studied on 36 patients. Readings were taken on admission (T-1), at which time a Barthel Index Mobility Goal (BIMG) was assigned, and again 4 to 8(More)
The action of various feedback modifiers on Bacillus stearothermophilus glutamine synthetase has been investigated by initial velocity kinetics, using the Mn2+-stimulated biosynthetic assay at 55 degrees C. The most potent inhibitors, used singly, are AMP, L-glutamine, and L-alanine. Other modifiers of significance include glycine, CTP, L-histidine,(More)
The question of how the supinator syndrome and the posterior interosseous syndrome are (or are not) related has not been well discussed in the literature. The anatomy of the radial nerve and its innervations is quite variable, as are the etiology, presentation, and clinical findings in the lesions of the posterior interosseous nerve. The present study was(More)
  • 1