J. C. de Man

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  • J. C. de Man
  • European journal of applied microbiology and…
  • 2005
A method is described for drawing up MPN tables restricted to results with a defined minimal probability and containing 95 and 99% confidence limits calculated on the basis of probability histograms. Tables for 3, 5 and 10 tubes, respectively, in three decimal dilutions and also a table for 5 tubes in the first dilution and 1 tube in two decimal dilutions(More)
Particles resembling virions observed in brain biopsies of two patients with Creutzfeldt-Jakob disease were located almost exclusively in the cytoplasm of the astrocytes which were swollen and showed accumulations of glia fibres, sometimes the cell bodies of the astrocytes were destroyed. Virusähnliche Partikel wurden in Gehirnbiopsien von zwei Patienten(More)
A new method for the demonstration of adenosine triphosphatase (ATP'ase) activity in tissue blocks with the electron-microscope has been developed. This method consists of short fixation (2–3 minutes,ph 7.3) in a OsO4-Tris-HCl mixture, incubation with a medium containing Tris-HCl buffer, tartrate, lead and magnesium ions, ATP and sucrose (ph 6.8) and(More)
To investigate the multivalent effect for up-regulating the intracerebral delivery of nanoparticles via receptor-mediated transcytosis. Nanoparticles labeled with near-infrared (NIR) fluorophore and different numbers of angiopep-2 peptides that specifically target low-density lipoprotein receptor-related protein (LRP) on the brain capillary endothelial(More)
The fine structural morphology and the ATP-ase activity of heart muscle at various sarcomere lengths was studied. ATP-ase activity was found on endothelial cells, cell membrane, intercalated disc, sarcoplasmic reticulum and in the Z-line region. It appeared that the activity of the ATP-splitting in the Z-line region was sensitive to PCMB and could be(More)
From the results of the experiments described, as well as from the literature, it becomes clear that the definition ofLactobacillus bulgaricus given in Bergey's Manual should be altered. This bacterium produces laevorotatory (D) lactic acid almost exclusively and ferments a limited number of sugars only,e.g. not maltose, saccharose or trehalose. All strains(More)
The amount of acid formed in grass silage was greater than could have been formed from the soluble sugars present, even when only a lactic fermentation took place. This seemed to point to fermentation of cell wall substances by lactic acid bacteria. Lactic acid fermentation in potato pulp always takes place with cell wall substances as substrates, as sugars(More)
Electron microscopy of untreated ascites tumour revealed that in many cells a juxta-nuclear area was found lying closely adjacent to the eccentrically located nucleus. This area consisted of well developed membranes and vesicles. R.N.P. particles and mitochondria were practically absent in this area. Virus-like particles were found mostly in this area.(More)