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Functioning gonadotropin-releasing hormone (GnRH) receptor is visualized as an aggregate of identical subunits (not all always functional) with the aggregate usually transformed into at least four successive structurally distinct receptor assemblies. Receptor protein, hormone molecule(s), and carrier(s) are main components of each functional subunit. During… (More)
In a hormonal system stimulated by one of the known hypothalamic peptides, gonadotropin releasing hormone (GnRH), the biological response seen, when physiologically called for, may be positively co-operative, showing as well the expected dynamics due to a singular or continuing stimulus. Mediation is by a receptor protein which in purified form shows… (More)
LH release from the anterior pituitary was studied by the method of an in vitro superfusion of bovine anterior pituitary tissue slices. LH release was stimulated by increased potassium concentration (23 and 59 mM) and by synthetic GnRH (1 and 4 ng/ml). While the potassium effect was completely dependent on extracellular calcium, that of GnRH was only… (More)
Luteinizing hormone (LH) release and tissue content were studied in explanted bovine pituitaries from heifer and steer during superfusion in vitro. Compared to steer, heifer pituitaries contained significantly more LH and released more LH into the incubation medium. Quantitative response in LH release to gonadotropin releasing hormone (GnRH) stimulation was… (More)