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Epidemiological and environmental surveys in the Cappadocian region of Turkey have linked the high incidence of pleural and peritoneal mesothelioma in the occupants of some villages with the zeolite fibres released from the locally occurring volcanic tuff. In view of the low ambient fibre concentrations and the extraordinary incidence of mesothelioma a(More)
In the present study, we investigated structural and functional aspects of stress fibers in a cell type in situ, i.e., the sinus endothelium of the human spleen. In this cell type, stress fibers extend underneath the basal plasma membrane and are arranged parallel to the cellular long axis. Ultrastructurally, the stress fibers were found to be composed of(More)
Malaria is a major cause of global morbidity and mortality, and new strategies for treating and preventing this disease are needed. Here we show that the Streptococcus pyogenes Cas9 DNA endonuclease and single guide RNAs (sgRNAs) produced using T7 RNA polymerase (T7 RNAP) efficiently edit the Plasmodium falciparum genome. Targeting the genes encoding native(More)
Most current computer architectures use a high−speed cache to translate user virtual addresses into physical memory addresses. On machines that require software to implement cache fills and invalidations, the software task is fairly straightforward. In a multi−processor multi−cache configuration, however, where processes are allowed to migrate across(More)
The available tools for conditional gene expression in Plasmodium falciparum are limited. Here, to enable reliable control of target gene expression, we build a system to efficiently modulate translation. We overcame several problems associated with other approaches for regulating gene expression in P. falciparum. Specifically, our system functions(More)
The construction of plasmid vectors for transgene expression in the malaria parasite, Plasmodium falciparum, presents major technical hurdles. Traditional molecular cloning by restriction and ligation often yields deletions and re-arrangements when assembling low-complexity (A + T)-rich parasite DNA. Furthermore, the use of large 5′- and 3′- untranslated(More)
Standard (UICC) crocidolite was subjected to ball milling to reduce the length of the fibre. These milled materials and the original standard sample were injected into the pleural cavity of rats to determine their ability to induce mesothelioma. Previous in vitro work on the same materials had suggested that biological activity was related to fibres greater(More)
PLEURAL MESOTHEILOMAS are known to be associated with exposure to asbestos in man (Wagner et al. 1960). Intrapleural injection of asbestos has been shown to produce mesotheliomas in rats (Wagner & Berry, 1969). By exploiting this model we have shown that these tumours may be transplanted s.c. into syngeneic rats (Brown et al., 1980). Seven such tumours have(More)
EXPERIMENTAL and pathological investigations have shown asbestos to be associated with mesothelioma in animals and man (Wagner & Berry, 1969; Wagner et al., 1960). In man the development of this neoplasm can take as long as 30-40 years after the first exposure to asbestos (Selikoff & Lee, 1978) and a comparable long latency period (18 months to 2 years) is(More)