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Phosphodeoxyribomutase from Escherichia coli has been purified to homogeneity. Chromatography on DEAE-Sephadex revealed three peaks of enzyme activity, designated form I, form II, and form III. Form III could be further separated into form III-1 and form III-2 by polyacrylamide gel electrophoresis. The four different molecular forms of the enzyme thus(More)
Synchronised cells of Tetrahymena pyriformis GL were labelled with 3H thymidine at a stage in the cell cycle when only the mitochondrial and extrachromosomal nucleolar ribosomal DNAs were replicating. In this way it was possible to prepare nuclei labelled selectively in the DNA of the ribosomal RNA genes. Since the ribosomal RNA cistrons of these cells are(More)
Replicative intermediates of discrete size (approximately 41 S) are observed in the eukaryotic organism Tetrahymena pyriformis, when the organism is grown under defined physiological conditions. The intermediates (believed to represent replicons) are synthesized and accumulated over longer periods of time (less than 90 min), if the cells are treated with(More)
In order to evaluate the solenoid actuator's reliability characteristics, we performed the following four-step approaching methods : (1) Firstly, the main test items decided through 2-stage QFD after analysis of the cause and effect of major failure modes through FMEA and FTA (2) The performance test was carried out by use of five sample solenoid actuators(More)
The extrachromosomal DNA coding for the ribosomal precursor in Tetrahymena contains a transcribed region with a size of 6 x 10(3) base pairs plus non-transcribed central and distal spacers. In the present study the chromatin structure of the transcribed region and the terminal spacer have been compared. Micrococcal nuclease and DNase I were used to(More)
Nucleoli can be isolated from Tetrahymena in a yield of 30-60%. The isolated nucleoli contain rDNA (at least 90% pure) and have a protein to DNA ratio of 30:1. The endogenous RNA-polymerase activity of the r-chromatin has the following properties: (i) The in vitro transcript has a maximal size identical to the in vivo 35S rRNA precursor, demonstrating(More)