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A method is proposed for a key safeguarding scheme sacrificing some of this sharpness if there were compensat(threshold scheme) in which the shadows are congruence classes of a number associated with the original key. A variation of this scheme ing improvements in some other feature, e.g., speed. provides efficient error detection and even exposes(More)
Type I restriction enzymes use two motors to translocate DNA before carrying out DNA cleavage. The motor function is accomplished by amino-acid motifs typical for superfamily 2 helicases, although DNA unwinding is not observed. Using a combination of extensive single-molecule magnetic tweezers and stopped-flow bulk measurements, we fully characterized the(More)
Type I restriction enzymes bind sequence-specifically to unmodified DNA and subsequently pull the adjacent DNA toward themselves. Cleavage then occurs remotely from the recognition site. The mechanism by which these members of the superfamily 2 (SF2) of helicases translocate DNA is largely unknown. We report the first single-molecule study of DNA(More)
The Type I restriction-modification enzyme EcoR124I is an archetypical helicase-based dsDNA translocase that moves unidirectionally along the 3'-5' strand of intact duplex DNA. Using a combination of ensemble and single-molecule measurements, we provide estimates of two physicochemical constants that are fundamental to a full description of motor protein(More)
Simulation tools are more and more used during the development and optimization of ultrasonic inspection techniques. Their advantages are clear. They give additional insight in the performance of an inspection technique at low cost. Furthermore, they overcome practical limitations like availability of transducers and test blocks during the design of new(More)
Most ultrasonic tools for the inspection of pipelines or bore-holes consist of a transducer, separated from the exterior by an acoustic window. This acoustic window protects the device from the potentially hostile exterior and is part of the mechanical construction. Therefore the acoustic window is positioned between the transducer and the region of(More)
Determination of the reinitiation time We define a single translocation event as the time from when the DNA end-to-end distance starts to decrease due to translocation activity until the point where the end-toend distance returns to its resting length due to termination of translocation. Considering only one of the translocation directions of a(More)
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