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Several preclinical and clinical studies have documented that dose or dose intensity of chemotherapeutic agents are important factors for response of patients' tumors. This finding has prompted empiric trials of certain chemotherapeutic agents in high-dose or regional administration treatment regimens. The present study was performed to identify agents that(More)
Polypeptide growth factors play an important role in the in 'itr)o growth of many human cancer cells (Rozengurt, 1983; Goustin et al., 1986). Recently, factors have been identified which are unique in their ability to induce a transformed phenotype on normal cells in culture (DeLarco & Todaro, 1978). Exposure of cells to these factors causes loss of(More)
Polypeptide growth factors bind to membrane receptors on human breast cancer cells and stimulate cell proliferation, suggesting that they may be important in growth regulation. Inhibition of the stimulatory effects of these factors might result in antineoplastic activity. Since cytotoxic drugs have been shown to alter cell membrane characteristics, we have(More)
As now constituted, the human tumor cloning assay performed in Petri dishes has several limitations including: (a) not all patients' tumors form colonies in the assay; (b) the plating efficiencies (number of colonies formed/number of cells plated) are low; and (c) a large number of tumor cells are required to perform drug sensitivity testing. In this study(More)
Transforming growth factors (TGF) are polypeptides that stimulate anchorage-independent growth of various nontransformed cells in vitro. Transforming growth factors have been found in tumor extracts and in the urine of cancer patients. The specific questions of our study were whether TGF activity can be detected in malignant effusions, how different assays(More)
We have evaluated a semiautomated radiometric assay (BACTEC 460 system) for screening of activity of anticancer drugs against human non-small cell lung cancer cell lines. Cells from seven cell lines were exposed to standard antineoplastic agents at four different concentrations using a 1-h incubation. Alpha 2-interferon was tested using a continuous(More)
alpha-Transforming growth factors (TGFs) are low-molecular-weight polypeptides (Mr 5000-7000) which are secreted by a variety of human cancer cells in vitro. Their presence has also been reported in the urine of patients with malignancies, human tumor extracts, and in the conditioned medium of primary human tumor cell cultures. There is evidence that(More)
Transforming growth factor-alpha (TGF alpha) is a low molecular weight peptide that has been implicated in an autocrine growth-stimulation of tumor cell lines in vitro. Although its mRNA has been detected in biopsies from human malignancies, the importance of an autocrine mechanism for the growth of human malignancies in vivo is unknown. The purpose of our(More)
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