J A Traas

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We have studied the F-actin network in cycling suspension culture cells of carrot (Daucus carota L.) using rhodaminyl lysine phallotoxin (RLP). In addition to conventional fixation with formaldehyde, we have used two different nonfixation methods before adding RLP: extracting cells in a stabilizing buffer; inducing transient pores in the plasma membrane(More)
Because two division planes form at right angles, male meiosis in higher plants provides striking examples of both division control and spatial programming. To investigate these processes we have stained microtubules and actin filaments during male mei-osis in the eggplant. Our results indicate the following. (1) That microtubules and their nucleation sites(More)
Cortical microtubule arrays in meristematic and differentiated cortical cells from root tips of Raphanus sativus were studied using both immunofluorescence and dry cleaving. Length, density and orientation of the cortical microtubules were measured. Between individual, non-dividing cells of the meristematic zone the mean microtubule length varied from 0.9(More)
A method is described for localizing microtubules using gold-labeled antibodies in combination with anti-tubulin. Cortex cells of Equisetum hyemale are broken open while still in buffer, after initially being attached to poly-L-lysine-coated grids. Thus, the cytoplasm becomes accessible to the antibodies. After application of the antibodies, the cleaved(More)
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