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BACKGROUND The objective of the present study is to investigate the migration pattern of the splenic dendritic cell of the chicken named the ellipsoid-associated cell (EAC) from the site of initial location at the periphery of the ellipsoid to the splenic T- and B-dependent areas. METHODS Bovine serum albumin bound to biotin and conjugated to gold(More)
The avian follicular dendritic cell changes that occur in the germinal center of the Harderian gland during the course of the immune response were studied by electron microscopy and the immunoperoxidase method was employed for the detection of S-100 protein. The chickens were injected twice with Salmonella O Antigen into the nictitating membrane at 9-day(More)
In the present study S-100 protein containing cells in the caecal tonsil were investigated, both at light microscopic and at electron microscopic levels, after oral coccidia inoculation (Eimeria tenella). The birds were infected with a single (Day 0) or two (Days 0 and 21) infective doses of 500 oocysts. Immunoelectron reactivity for S-100 protein was(More)
A 6-year-old, sterile, Blanca Celtibérica breed adult doe was referred to our faculty. The doe had external female genitalia, a short anogenital distance, and normally shaped udders. Masculinization signs in the head shape and male behavior were also noted at the time of referral. Genetic analysis demonstrated normal 2n = 60 XX karyotype and an absence of(More)
BACKGROUND There is a need to identify the follicular dendritic cells (FDC) of the chicken spleen at the ultrastructural level during a secondary immune response. METHODS The cells were identified after intravenous priming BSA and boosting with biotinylated BSA conjugated to colloidal gold particles. Monoclonal antibodies raised specifically either to(More)
BACKGROUND In the chicken, circulating antigens enter the splenic white pulp via the Schweigger-Seidel sheaths (ellipsoids), where they are bound by cells, the ellipsoid associated cells (EAC), which are located on the periphery of the ellipsoid. There is an increasing body of evidence that these antigen-binding cells move through the PALS, to be finally(More)
Histopathological changes were observed in 10 ewe lambs reared during nine months on lucerne as compared with 10 controls reared on ryegrass. These changes consisted of periglandular oedema and subacute endometritis in the uterus, secretory-cell hyperplasia with prevalence of neutral mucopolysaccharides in the cervix and hyperkeratinisation and colpitis in(More)
BACKGROUND The bursa of Fabricius provided the microenvironment for B-cell differentiation. Continuous contact between lymphoid cells and antigen in the bursa further suggested that antigenic material has an important influence on the maintenance and development of B cells in the bursa. In addition, a dendritic cell, the bursal secretory dendritic cell(More)
The immunoglobulin (Ig) levels in tears and sera were compared after antigen administration (salmonella O antigen) by eyedrop and injection into the nictitating membrane, to determine the Ig classes synthesised by the plasma cells in the chicken Harderian gland. Samples of tears and sera were collected from immunised and control birds between 24 hours and(More)
An experimental study was made of 20 dogs in order to compare various surgical techniques used to correct eversion of the third eyelid, namely resection of most of the cartilage, resection of the central portion of the cartilage, and cartilage homotransplantation. An analysis was made of histological results obtained 45 days after operation, the most(More)