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Electron microscopy and single-particle analyses have been carried out on negatively stained photosystem II (PSII) complexes isolated from the green alga Chlamydomonas reinhardtii and the thermophilic cyanobacterium Synechococcus elongatus. The analyses have yielded three-dimensional structures at 30-A resolution. Biochemical analysis of the C. reinhardtii(More)
Single particle analysis and 3D reconstruction of molecules imaged by transmission electron microscopy have provided a wealth of medium to low resolution structures of biological molecules and macromolecular complexes, such as the ribosome, viruses, molecular chaperones and photosystem II. In this review, the principles of these techniques are introduced in(More)
Paraffin-embedded human and animal tissues were stained with spectrally pure thionine, 1,3-diaminophenthiazoniumchlorate and 1,7-diaminophenthiazoniumperchlorate. They were examined by light microscopy to determine their applicability in histologic diagnosis. Furthermore the effect of salts and of the type of dye solvent on the staining properties of(More)
The staining properties of 4 spectral pure derivates of Phenoxazin--Capriblue GN, Stella Blue, Oxonin, and Punky Blue--were investigated using human and animal tissues. Punky Blue and Stella Blue are newly synthesized derivates. A simplified staining technique was developed based on conventional fixing and mounting methods of histological materials. Punky(More)
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