Július Rajčáni

Learn More
UNLABELLED The anti-apoptotic protein survivin was detected in a panel of 27 dysplastic nevi. From each representative paraffin block 4 mm sections were cut and stained with anti-survivin antibody (DAKO, Clone 12C4). In each section, the labeling intensity, the subcellular location of survivin antigen, the percentage of labeled cells and the degree of(More)
Newborn white mice were susceptible to peroral (p.o.) infection with murine alphaherpesvirus isolated from free-living Clethrionomys glareolus. Death occurred within 6-8 days in animals infected with the higher virus dose of 4.8 log10 TCID50. Clinical symptoms also occurred in some animals infected with lower doses, while others developed inapparent(More)
The herpes simplex virus (HSV) has a 152 kbp dsDNA encoding probably 84 proteins. The approximate number of ORFs is 94, from which seven are doubled. The most probable number of single copy ORFs is 84 after omitting the two latency associated transcripts (LAT)/ORFs and the putative UL27.5 ORF. The high gene number creates a “crowded” genome with several(More)
Murine herpes virus (MHV), a natural pathogen originally isolated from free-living rodents, constitutes the most amenable animal model for human gamma herpesviruses. Based on DNA sequence homology, this virus was classified as Murid Herpesvirus 4 to subfamily Gammaherpesvirinae. Pilot studies in our laboratory, using mice inoculated by the intranasal route,(More)
Latent infection with the TOP strain of pseudorabies virus (PRV) was established in 6 weeks old piglets. Infectious virus was found in oropharyngeal swabs till day 10 post infection (p.i.); later on, attempts to detect the virus remained unsuccessful. However, PRV could be isolated by explantation of tonsils, cervical lymph nodes, nasal mucosa and gasserian(More)
During the last decade, new data accumulated describing the early events during herpes simplex virus 1 (HSV-1) replication occurring before capsid formation and virion envelopment. The HSV virion carries its own specific transcription initiation factor (α-TIF), which functions together with other components of the cellular transcriptase complex to mediate(More)
Antibodies to herpes simplex virus type 1 (HSV-1) were tested in the cerebrospinal fluid (CSF) of 262 schizophrenic patients by virus neutralization test (VNT) and enzyme-linked immunosorbent assay (ELISA). While VNT in the presence of complement revealed antibodies to HSV-1 in 18.3% of samples, ELISA was positive in 61.2% of cases; both tests were positive(More)
We followed the kinetics of reactivation of latent Herpes simplex virus 1 (HSV-1) infection established in rabbits by corneal route. The corresponding trigeminal ganglia (TG) were cultured and the culture medium was examined at daily intervals for release of infectious virus. Sections from the cultured TG fragments were stained with antisera against(More)