Jörn Splinter

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DNA double-strand breaks (DSBs) can induce chromosomal aberrations and carcinogenesis and their correct repair is crucial for genetic stability. The cellular response to DSBs depends on damage signaling including the phosphorylation of the histone H2AX (γH2AX). However, a lack of γH2AX formation in heterochromatin (HC) is generally observed after DNA damage(More)
We studied the spatiotemporal organization of DNA damage processing by live cell microscopy analysis in human cells. In unirradiated U2OS osteosarcoma and HeLa cancer cells, a fast confined and Brownian-like motion of DNA repair protein foci was observed, which was not altered by radiation. By analyzing the motional activity of GFP-53BP1 foci in live cells(More)
The induction of localized DNA damage within a discrete nuclear volume is an important tool in DNA repair studies. Both charged particle irradiation and laser microirradiation (LMI) systems allow for such a localized damage induction, but the results obtained are difficult to compare, as the delivered laser dose cannot be measured directly. Therefore, we(More)
Irradiation of cell nuclei with charged particles leads to the spatially defined production of DNA damage along the particle trajectories, thus facilitating studies on the dynamics of radiation-induced protein foci associated with lesion processing. Here we used visual inspection and computational analysis of the track morphology after immunodetection to(More)
We studied the spatiotemporal organization of DNA damage processing by live cell microscopy analysis of GFPtagged 53BP1in human cells after the exposure to charged particles. The inhomogeneous dose deposition along the ion trajectories facilitates studies on the dynamics of radiation-induced protein foci [1,2]. In unirradiated U2OS osteosarcoma cells, a(More)
DNA is highly compacted by wrapping around histones and folding into higher order structures building chromatin. This chromatin can be subdivided in two classes: the highly transcriptional active, sparse euchromatin and the densely packed heterochromatin. The heterochromatin is transcriptionally inert but essential for the regulation of the gene expression(More)
In the last decade an increasing number of reports are published in which UV laser micro irradiation (UVLM) is used as a new tool to generate DNA double-strand breaks (DSBs) in localized regions within single nuclei [1]. Until then only heavy ions (HI) were known for their ability to induce DSBs in strictly localized areas of cell nuclei [2], but compared(More)
Exposure of mammalian cell nuclei with charged particles leads to the spatially defined production of damaged chromatin domains along the particle trajectories. Analysis of track morphology facilitates studies on the dynamics of radiation-induced protein foci associated with lesion processing. Recently we described the discrete formation of γ-H2AX foci and(More)
DNA is compacted into chromosomes in the nucleus of cells. The ends of these chromosomes (telomeres) are associated to proteins to form a dynamic cap that protects the DNA from being viewed as double-strand breaks (DSBs) and eliciting a DNA damage response. One of the proteins responsible for telomere capping is TRF2 (telomeric repeat binding factor 2).(More)
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