Jörg Neukammer

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This study demonstrates the suitability of microfluidic structures for high throughput blood cell analysis. The microfluidic chips exploit fully integrated hydrodynamic focusing based on two different concepts: Two-stage cascade focusing and spin focusing (vortex) principle. The sample--A suspension of micro particles or blood cells--is injected into a(More)
The scope of this paper is to illustrate the need for an improved quality assurance in fluorometry. For this purpose, instrumental sources of error and their influences on the reliability and comparability of fluorescence data are highlighted for frequently used photoluminescence techniques ranging from conventional macro- and microfluorometry over(More)
A surface-labeled lyophilized lymphocyte (sLL) preparation has been developed using human peripheral blood mononuclear cells prelabeled with a fluorescein isothiocyanate conjugated anti-CD4 monoclonal antibody. The sLL preparation is intended to be used as a reference material for CD4+ cell counting including the development of higher order reference(More)
Reference measurement procedures are indispensable to reliably determine blood cell concentrations, since medical diagnosis, initiation of therapy and control of blood products are often based on concentration limits of specific cell subpopulations. We have developed a reference procedure to directly determine stem cell concentrations. In the same(More)
A method is described to determine cell concentrations for erythrocytes and leukocytes with typical uncertainties of 0.8% and 2%, respectively. The unique method is designed in such a way that results are traceable to SI derived units. The sample suspensions are prepared under gravimetric control. The sample volume measured in cell counting is determined by(More)
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