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We report the direct experimental observation of photonic nanojets created by single latex microspheres illuminated by a plane wave at a wavelength of 520 nm. Measurements are performed with a fast scanning confocal microscope in detection mode, where the detection pinhole defines a diffraction-limited observation volume that is scanned in three dimensions(More)
Dielectric microspheres are shown to be capable of confining light in a three-dimensional region of subwavelength dimensions when they are illuminated by tightly focused Gaussian beams. We show that a simple configuration, not involving resonances, permits one to reach an effective volume as small as 0.6 (lambda/n)(3). It is shown that this(More)
Quantum continuous variables are being explored as an alternative means to implement quantum key distribution, which is usually based on single photon counting. The former approach is potentially advantageous because it should enable higher key distribution rates. Here we propose and experimentally demonstrate a quantum key distribution protocol based on(More)
We detail the role of single nanometric apertures milled in a gold film to enhance the fluorescence emission of Alexa Fluor 647 molecules. Combining fluorescence correlation spectroscopy and lifetime measurements, we determine the respective contributions of excitation and emission in the observed enhanced fluorescence. We characterize a broad range of(More)
Received (received date) Revised (revised date) We discuss quantum key distribution protocols using quantum continuous variables. We show that such protocols can be made secure against individual gaussian attacks regardless the transmission of the optical line between Alice and Bob. This is achieved by reversing the reconciliation procedure subsequent to(More)
Light/dark regulation of the Calvin cycle in oxygenic photosynthetic organisms involves the formation and dissociation of supramolecular complexes between CP12, a nuclear-encoded chloroplast protein, and the two enzymes glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (EC and phosphoribulokinase (PRK) (EC Despite the high importance of(More)
We report the experimental proof of molecular count rate enhancement (up to 6.5-fold) and lifetime reduction for single fluorescent molecules diffusing in subwavelength apertures milled in aluminum films. The observed enhancement dependence with the aperture diameter agrees qualitatively with numerical electromagnetic computations of the excitation power(More)
Recent advances in nanophotonics open the way for promising applications towards efficient single molecule fluorescence analysis. In this review, we discuss how photonic methods bring innovative solutions for two essential questions: how to detect a single molecule in a highly concentrated solution, and how to enhance the faint optical signal emitted per(More)
We describe the development of a new methodology to probe the plasma membrane organization of living cells at the nanometric scale. Single nanometric apertures in a metallic film limit the observed membrane area below the optical diffraction barrier. The new approach performs fluorescence correlation spectroscopy with increasing aperture sizes and extracts(More)
Fluorescence Correlation Spectroscopy is used to investigate fluorescent molecules in solution diffusing in subwavelength rectangular apertures milled in Aluminium films. This rectangular shape allows to switch between a propagating and an evanescent excitation field within the aperture, leading to a significant tunability of the observation volume. Due to(More)