Jérôme R E Del Castillo

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A rapid method is described for the simultaneous preparation of both membranes of guinea-pig enterocytes, using simple differential centrifugation techniques. Basolateral membranes were purified on a Percoll gradient and the final yield of (Na+ + K+)-ATPase was 12.4% of the original activity with an enrichment factor of 12.6-fold. Purification of the(More)
By the end of 2004, the Canadian swine population had experienced a severe increase in the incidence of Porcine circovirus-associated disease (PCVAD), a problem that was associated with the emergence of a new Porcine circovirus-2 genotype (PCV-2b), previously unrecovered in North America. Thus, it became important to develop a diagnostic tool that could(More)
Due to renal COX-2 constitutive expression, meloxicam is presumably deleterious for kidney function in critical situations. The present study investigates the influence of intravenous meloxicam on renal parameters and compares it with a nonselective COX inhibitor, ketoprofen. Piglets (n = 6 in each group) were treated with ketoprofen (2 mg.kg(-1)),(More)
This study aimed to establish the effect of a diet enriched with green-lipped mussel (GLM) on pain and functional outcomes in osteoarthritic dogs. Twenty-three client-owned dogs with osteoarthritis (OA) were fed a balanced control diet for 30 d and then a GLM-enriched balanced diet for the next 60 d. We assessed peak vertical force (PVF), which is(More)
Pharmacokinetic-pharmacodynamic (PK/PD) surrogate indices (AUIC, AUC/MIC, C(max)/MIC, T>MIC) for measuring antibiotic efficacy are presented and reviewed. As clinical trials are not sufficiently sensitive to establish a dosage regimen which guarantees total bacteriological cure (Pollyanna phenomenon), PK/PD indexes have been proposed from in vitro, ex vivo,(More)
Variability in drug intake is increasingly recognized as a major source of variability in drug response. The non-uniform access to medicated feed, influenced by swine individual feeding behaviour, is a determinant of antibiotic exposure, recalling the intrinsic similarity with human compliance to drug regimens. In this paper, we developed a feeding(More)
PURPOSE Because oxytocin (OT) is potentially useful in cardiovascular therapy but has hormonal roles on the cardiovascular and renal systems, we characterized its pharmacokinetic (PK) properties as a function of dose. METHODS A single intravenous bolus of OT was given at doses of 200, 300, 500, 1000, 3000, 5000 and 10000 ng/kg to anesthetized male rats (n(More)
BACKGROUND Bone marrow aspiration (BMA) is a clinical procedure frequently performed in dogs. OBJECTIVE To compare levels of pain intensity induced by 3 different BMA procedures using several pain scoring instruments. ANIMALS Sixteen healthy Beagles. METHODS A prospective experimental pilot study was conducted using blinded observers. Dogs were(More)
OBJECTIVE To compare glomerular filtration rate (GFR) as estimated from Patlak plot analysis by use of single-slice computed tomography (CT) with that obtained from clearance of plasma inulin in pigs. ANIMALS 8 healthy anesthetized juvenile pigs. PROCEDURES All pigs underwent precontrast, whole-kidney, helical CT; postcontrast single-slice dynamic CT;(More)