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OSprof is a versatile, portable, and efficient profiling methodology based on the analysis of latency distributions. Although OSprof has offers several unique benefits and has been used to uncover several interesting performance problems, the latency distributions that it provides must be analyzed manually. These latency distributions are presented as… (More)
We have developed an assay system for endo-beta-N-acetylglucosaminidase and glycoamidase (PNGase), using Eu(3+)-labeled Man(9)GlcNAc(2) glycopeptides as substrates in combination with lectin capture. Two glycopeptides of different peptide lengths, derived from soybean agglutinin, were labeled with Eu(3+) via a diethylenetriaminepentaacetate (DTPA) chelating… (More)
The title compound was prepared by enzymatic transfer of oligosaccharide to a synthetic pentapeptide containing the Glc-Asn linkage. The compound was not hydrolyzed by glycoamidases from plant and bacterial sources, but it inhibited both enzymes in the micromolar range. Its activity is compared to other potential inhibitors.
Some larger high-mannose-type oligosaccharides bind very tightly to concanavalin A and are difficult to elute. We present conditions that permit the complete elution of compounds containing high-mannose type oligosaccharides from a popular concanavalin A-Sepharose that is commercially available. Europium-labeled Man9GlcNAc2Asn (N. Kawasaki and Y.C. Lee,… (More)