Iu L Degtiarev

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To express HIV-1 reverse transcriptase in E. coli a number of genetic constructions containing reverse transcriptase and virus protease nucleotide sequences was obtained. The products of expression were characterized; monoclonal antibodies to reverse transcriptase were produced. The purification of reverse transcriptase was carried out. The substantial(More)
Conditions of the enzyme immunoassay for the detection of HSV-I by the "sandwich"-method at all stages of the testing were optimized using monoclonal antibodies (monoAB). When identical concentrations of monoclonal antibodies and durations of their adsorption on polystyrene plates were used, the signal in EIA (A405) with carbonate buffer was by 30% higher(More)
The monoclonal antibodies to DNA-dependent RNA-polymerase of bacteriophage T7 have been obtained. Twenty of the obtained 500 clones have inhibited the enzyme activity. Three specificity groups were identified for seven of the clones supporting their affinity for different antigenic determinants.
Four clones producing monoclonal antibodies inhibiting enzymatic activity were used to localize the functionally important antigenic determinants of T7 RNA polymerase. All antibodies were shown to bind to C-terminal fragment of the protein (residues 589-883). The competition studies showed the specificity of the three antibodies toward one epitope and the(More)
Studies were carried out aimed at isolation of the causative agent of ornithosis from the organs of pigeons and budgerigars. The present study was based on the results of a serological survey which had revealed a high rate of infection with ornithosis in pigeons as well as a case of ornithosis in an owner of budgerigars. The isolates were identified, their(More)
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