Isobel Parkin

Margaret Y. Gruber2
Horace Wei1
2Margaret Y. Gruber
1Horace Wei
1Yu-Zhen Xi
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A new method to improve the efficiency of flanking sequence identification by genome walking was developed based on an expanded, sequential list of criteria for selecting candidate enzymes, plus several other optimization steps. These criteria include: step (1) initially choosing the most appropriate restriction enzyme according to the average fragment size(More)
Complex T-DNA repeat structures are a frequent outcome of Agrobacterium-mediated plant transformation and often lead to co-suppression of gene expression, even gene silencing. Thus, they are undesired in transgenic plants for commercial or research purposes and frequently need to be efficiently identified in a large transgenic plant population. Application(More)
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