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The scarcity of usable nitrogen frequently limits plant growth. A tight metabolic association with rhizobial bacteria allows legumes to obtain nitrogen compounds by bacterial reduction of dinitrogen (N2) to ammonium (NH4+). We present here the annotated DNA sequence of the alpha-proteobacterium Sinorhizobium meliloti, the symbiont of alfalfa. The tripartite(More)
Two genes encoding citrate synthase, a key enzyme in the Krebs cycle, have been found in Rhizobium tropici. One of them is in the bacterial chromosome, while the other is in the symbiotic plasmid. We sequenced the chromosomal gene and found that it is very similar to the previously reported plasmidic gene sequence in its structural region but not in its(More)
Sinorhizobium sp. strain BR816 possesses two nodPQ copies, providing activated sulfate (3'-phosphoadenosine-5'-phosphosulfate [PAPS]) needed for the biosynthesis of sulfated Nod factors. It was previously shown that the Nod factors synthesized by a nodPQ double mutant are not structurally different from those of the wild-type strain. In this study, we(More)
The glyoxylate cycle is an anaplerotic pathway of the tricarboxylic acid cycle that allows bacteria to grow using acetate, fatty acids, or poly-β-hydroxybutyrate (PHB). In Sinorhizobium meliloti, activities of the glyoxylate cycle enzymes isocitrate lyase (AceA) and malate synthase (GlcB) are present during growth on these kinds of carbon sources, but a(More)
The alpha-protobacterium Rhizobium leucaenae CFN 299 is able to nodulate and fix nitrogen in symbiosis with a wide range of legumes, including Phaseolus vulgaris (common bean). Strain CFN 299 contains a 500-kb symbiotic plasmid (pSym) that encodes genes required for nodulation and nitrogen fixation as well as many genes whose function is unknown. In this(More)
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