Ishtiaq Ali Saaem

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Thermoplastic materials such as cyclic-olefin copolymers (COC) provide a versatile and cost-effective alternative to the traditional glass or silicon substrate for rapid prototyping and industrial scale fabrication of microdevices. To extend the utility of COC as an effective microarray substrate, we developed a new method that enabled for the first time in(More)
Exploiting the burgeoning fields of genomics, proteomics and metabolomics improves understanding of human physiology and, critically, the mutations that signal disease susceptibility. Through these emerging fields, rational design approaches to diagnosis, drug development and ultimately personalized medicine are possible. Personalized medicine and(More)
Low-cost, high-throughput gene synthesis and precise control of protein expression are of critical importance to synthetic biology and biotechnology. Here we describe the development of an on-chip gene synthesis technology, which integrates on a single microchip the synthesis of DNA oligonucleotides using inkjet printing, isothermal oligonucleotide(More)
Structural DNA nanotechnology, and specifically scaffolded DNA origami, is rapidly developing as a versatile method for bottom-up fabrication of novel nanometer-scale materials and devices. However, lengths of conventional single-stranded scaffolds, for example, 7,249-nucleotide circular genomic DNA from the M13mp18 phage, limit the scales of these uniquely(More)
Accurate, economical and high-throughput gene and genome synthesis is essential to the development of synthetic biology and biotechnology. New large-scale gene synthesis methods harnessing the power of DNA microchips have recently been demonstrated. Yet, the technology is still compromised by a high occurrence of errors in the synthesized products. These(More)
Steganography has become extremely popular lately as a means of achieving security for many multimedia applications. However, research on stego-attack mechanisms has also kept pace with this development. Robustness to such stego-attacks usually requires a compromise in stegocapacity. In this paper we propose a joint source-channel coding paradigm for attack(More)
The development of economical and high-throughput gene synthesis technology has been hampered by the high occurrence of errors in the synthesized products, which requires expensive labor and time to correct. Here, we describe an error correction reaction (ECR), which employs Surveyor, a mismatch-specific DNA endonuclease, to remove errors from synthetic(More)
This paper explores the use of surface-patterned nanohydrogels as a substrate for high-density and high-sensitivity protein arrays. Nanohydrogels were created by locally crosslinking dry amineterminated PEG 5000 thin films using a focused electron beam. Unirradiated polymer was subsequently washed away leaving behind gels approximately 200 nm in diameter(More)
The development of DNA microarray technology for high-throughput gene synthesis is hampered by poor sequence quality and inefficient gene assembly. We developed a programmable microchip using thermoplastic cyclic olefin copolymer (COC) functionalized with hydrophilic SiO2 thin film arrays, creating physically isolated picolitter reactors that constrain(More)