Isabelle Grasseau

Learn More
The need for semen preservation in domestic birds is a result of the reduction in genetic variability of domestic bird livestock and of the increasing risk of line extinction for health and safety reasons. Cryopreservation of embryos and primordial germ cells (PGC) is not routinely feasible in birds. The project therefore involved semen frozen in optimal(More)
Semen cryopreservation is very important for the ex situ management of genetic diversity in birds but it is rarely used. This is partly because of the highly variable success rates, and this emphasizes the need for predictors of semen freezability. This study evaluated the ability of semen quality tests to predict the success rates of semen cryopreservation(More)
This work demonstrates that spermatozoa from five avian species (chicken, turkey, guinea fowl, duck and goose) are all characterised by high proportions of polyunsaturated fatty acids, from 46 (turkey) to 55% (duck) of total. For each of the species, the most abundant fatty acids were arachidonic (20:4n-6) and docosatetraenoic (22:4n-6) acids, representing(More)
The ability to survive cryopreservation varies in spermatozoa from different bird species. Among the biological factors potentially responsible for such differences, species variations in membrane fluidity have a role in the restoration of the physiological state after freezing. Membrane fluidity may be assessed by measuring fluorescence polarization(More)
Broiler breeder roosters received two diets, containing either 5% salmon oil (SO) or 5% corn oil (CO). The diets differed essentially in their polyunsaturated fatty acid (PUFA) composition, with n-6:n-3 fatty acid ratios of 41.6 in SO and 1.5 in CO. The effects of these diets on the fatty acid composition of spermatozoa and seminal plasma, and on fertility(More)
Quantitative and qualitative changes may occur in the lipids of spermatozoa during in vitro storage of gametes and may indicate possible degradations occurring within the cells under these conditions. The aim of the present study was to investigate such changes. The motility, viability, morphological integrity and lipid content were measured in fowl semen(More)
Chicken spermatozoa may remain in the female oviduct for a prolonged period before induction of the acrosome reaction on contact with the inner perivitelline layer (IPVL). By contrast, the acrosome reaction may be induced very rapidly in vitro in the presence of IPVL and Ca(2+). In the present study, we examined the extent to which the chicken acrosome(More)
The effects of in vitro storage on the sperm's ability to undergo the acrosome reaction (AR) have never been studied in avian species despite its major importance for reproduction management. The ability of chicken sperm to undergo the AR was measured after liquid storage at 4 °C and after cryopreservation, and its relationship with other semen quality(More)
Sperm surviving after freezing-thawing is usually 40-50% of the initial population. Damage during this process affects both fertilizing ability and its duration in avian species. However, the effect of cryopreservation on the sperm ability to undergo the acrosome reaction, the initial event of fertilization, is still in question in birds. In this paper, the(More)
Currently, evaluation of sperm quality is primarily based on in vitro measures of sperm function such as motility, viability and/or acrosome reaction. However, results are often poorly correlated with fertility, and alternative diagnostic tools are therefore needed both in veterinary and human medicine. In a recent pilot study, we demonstrated that MS(More)