Isabelle Chevalot

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To design novel 3D in vitro co-culture models based on the RGD-peptide-induced cell self-assembly technique. Multicellular spheroids from M-3 murine melanoma cells and L-929 murine fibroblasts were obtained directly from monolayer culture by addition of culture medium containing cyclic RGD-peptide. To reach reproducible architecture of co-culture spheroids,(More)
Background Expansion of mesenchymal stromal cells (MSC) is one of the key steps for their use in tissue engineering or cell therapies. To increase cells expansion yields, two milestones have to be achieved that will allow a wider MSC therapeutic use, namely an optimal serum-free medium and a process intensification via 3D suspension culture on microcarriers(More)
Background Advantages of microencapsulation as a 3D growth system are chemically and spatially defined 3D network of extra-cellular matrix components, cell-to-cell and cell-to-matrix interactions governing differentiation, proliferation and cell function in vivo. The study is aimed at i) optimization of techniques for preparing microcapsules; ii) generation(More)
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