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The developmental potential of adult somatic nuclei after nuclear transfer (NT) into enucleated, in vitro-matured oocytes was evaluated in a dwarf breed of goat (BELE: Breed Early Lactate Early). Somatic donor cells were obtained from two different sources: 1) adult granulosa cells (GCs) and 2) fetal fibroblasts. Primary GCs were obtained from follicular(More)
This study evaluated the efficiency and toxicity of two cryopreservation methods, solid-surface vitrification (SSV) and cryoloop vitrification (CLV), on in vitro matured oocytes and in vivo derived early stage goat embryos. In the SSV method, oocytes were vitrified in a solution of 35% ethylene glycol (EG), 5% polyvinyl-pyrrolidone (PVP), and 0.4%(More)
This paper addresses the problem of super-resolving a single image and recovering the characteristics of the sensor using a learning-based approach. In particular, the point spread function (PSF) of the camera is sought by minimizing the mean Euclidean distance function between patches from the input frame and from degraded versions of high-resolution(More)
This paper presents comparisons of two learning-based super-resolution algorithms as well as standard interpolation methods. To allow quality assessment of results, a comparison of a variety of image quality measures is also performed. Results show that a MRF-based super-resolution algorithm improves a previously interpolated image. The estimated degree of(More)
The efficiency of laparoscopic ovum pick-up (LOPU) followed by in vitro embryo production (IVEP) in the propagation of aged goats with poor reproductive performance was evaluated in the present study. Follicular development was stimulated in donor goats with 80 mg follicle-stimulating hormone and 300 IU equine chorionic gonadotrophin administered 36 h(More)
This paper presents a reliable non-blind method to measure intrinsic lens blur. We first introduce an accurate camera-scene alignment framework that avoids erroneous homography estimation and camera tone curve estimation. This alignment is used to generate a sharp correspondence of a target pattern captured by the camera. Second, we introduce a Point Spread(More)
Dangerous organophosphorus (OP) compounds have been used as insecticides in agriculture and in chemical warfare. Because exposure to OP could create a danger for humans in the future, butyrylcholinesterase (BChE) has been developed for prophylaxis to these chemicals. Because it is impractical to obtain sufficient quantities of plasma BChE to treat humans(More)
BACKGROUND Human butyrylcholinesterase (huBChE) has been shown to be an effective antidote against multiple LD50 of organophosphorus compounds. A prerequisite for such use of huBChE is a prolonged circulatory half-life. This study was undertaken to produce recombinant huBChE fused to human serum albumin (hSA) and characterize the fusion protein. RESULTS(More)
Embryonic stem (ES) cells with the capacity for germ line transmission have only been verified in mouse and rat. Methods for derivation, propagation, and differentiation of ES cells from domestic animals have not been fully established. Here, we describe derivation of ES cells from goat embryos. In vivo-derived embryos were cultured on goat fetal fibroblast(More)