Isabel Rodrigues Gerhardt

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Departamento de Bioquı́mica, Universidade Federal do Rio Grande do Norte, 59072–970 Natal, Rio Grande do Norte, Brazil (M.P.S.); Departamento de Biologia Celular, Universidade de Brası́lia, Brasilia, Distrito Federal, Brazil (I.R.G.); Embrapa-Cenargen, Setor de Áreas Isoladas Norte Parque Rural, 70 710–900, Brasilia, Distrito Federal, Brazil (M.F.G.-d.-S.);(More)
Background Several factors may affect the genetic transformation efficiency of woody species. One factor is the use of an efficient selective agent that inhibits the development of non-transformed cells and just allows the development of transformed tissues. The most used selection agent is the neomycin phosphotransferase II (NPTII) gene, which confers(More)
The present study reports, for the first time in literature, the purification and biochemical characterization of a small basic protein from maize seeds similar to plant lipid transfer proteins-2, named mLTP2. The mLTP2 consists of 70 amino acid residues and has an M(r) of 7303.83, determined by electrospray ionization mass spectrometry. The primary(More)
Background In Brazil, especially in the Southern region, stresses caused by cold and eventual frost are those that exert the most negative effect on the productivity of Eucalyptus spp. The genetic transformation techniques may contribute to forestry improvement programs in order to obtain genotypes expressing new interesting characteristics. They allow(More)
Background Of the total worldwide biomass produced by land plants, 70% is represented by cell walls [1]. In angiosperm trees, the fixed carbon is accumulated mainly in the secondary walls of xylem cells, which turns xylem cell wall formation a major carbon sink in trees[2]. The understanding of mechanisms that regulate carbon flux to the synthesis of cell(More)
Background In vitro organogenesis is one of the key techniques associated with genetic transformation, as it determines the successful regeneration of transgenic plants after co-cultivation with bacteria. Therefore, the development of efficient regeneration protocols is the most critical step in developing genetic transformation. Protocols have been(More)
Departamento de Bioquı́mica, Universidade Federal do Rio Grande do Norte, 59072–970 Natal, Rio Grande do Norte, Brazil (M.P.S.); Departamento de Biologia Celular, Universidade de Brası́lia, Brasilia, Distrito Federal, Brazil (I.R.G.); Embrapa-Cenargen, Setor de Áreas Isoladas Norte Parque Rural, 70 710–900, Brasilia, Distrito Federal, Brazil (M.F.G.-d.-S.);(More)
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