Irina V Orlova

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The desC gene for the acyl-lipid Delta9-desaturase from the thermophilic cyanobacterium Synechococcus vulcanus was introduced into Nicotiana tabacum under control of the 35S promoter. Expression of the desaturase was confirmed by Western blotting. Lipid analysis revealed that lipid content and the extent of fatty acid unsaturation significantly increased in(More)
Tobacco plants were genetically transformed with the Arabidopsis thaliana heterologous hmg1 gene encoding 3-hydroxy-3-methylglutaryl-CoA reductase, a key enzyme involved in the metabolism of terpenoid compounds. The hmg1 gene was inserted under the control of the 35S RNA double promoter from the cauliflower mosaic virus (CaMV 35S) both in direct and reverse(More)
To date, many examples of successful expression of immunoglobulin variable domains, or miniantibodies (scFv), in plant cells are known. Simple requirements to assembling these molecules in the cell, the possibility of their targeted transport to different cell compartments, and the possibility of designing bifunctional antibodies on their basis facilitates(More)
Tobacco plants with the introduced desC gene for acyl-lipid Δ9-desaturase from the thermophilic cyanobacterium Synechococcus vulcanus were cultivated on agar-solidified Murashige and Skoog nutrient medium supplemented with ferulic acid and antibiotics at 22°C and a 16-h photoperiod. Control plants were transformed with an empty pGA482 vector. The analysis(More)
A recombinant scFv antibody against human spleen ferritin was expressed as a barstar-fused protein in Escherichia coli and in Nicotiana tabacum plants and suspension cell cultures. As demonstrated by immunoblotting with antibarstar antibodies, direction of the recombinant protein to the endomembrane system of plant cells ensured its stability and(More)
A recombinant scFv antibody against human spleen ferritin was expressed as a barstar-fused protein in Escherichia coli and in Nicotiana tabacum plants and suspension cell cultures. As demonstrated by immunoblotting with antibarstar antibodies, direction of the recombinant protein to the endomembrane system of plant cells ensured its stability and(More)
149 Environmental conditions experience periodic and random changes, and their deviations from normal conditions favorable for plant life attain frequently dangerous amplitudes. Temperature is one of the most limiting environmental factors controlling plant geographic spread and productivity. An importance of the problem of plant chilling tolerance is(More)
A comparative analysis of zono - and tomography in 69 patients with minor peripheral cancer of the lung (26), tuberculoma (24), hamartoma (19) was performed. Zonography was shown to improve differential diagnosis between them. However, its importance is limited and helps to better visualize changes of the lung tissue around the formations, "a path" outgoing(More)
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