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The effect of enzymatic pretreatment of sugar beet pulp and spent hops prior to methane fermentation was determined in this study. These industrial residues were subjected to enzymatic digestion before anaerobic fermentation because of high fiber content (of 85.1% dry matter (DM) and 57.7% DM in sugar beet pulp and spent hops, respectively). Their 24h(More)
The effect of Liquid Hot Water treatment conditions on the degree of sugar beet pulp (SBP) degradation was studied. The SBP was subjected to hydrothermal processing at temperatures ranging from 120 to 200 °C. The relationship between processing temperature and parameters of liquid and solid fractions of resulting hydrolysates as well as the efficiency of(More)
Xylanase of low molecular weight (K II) was isolated from the fungus Aspergillus niger IBT-90 cultivated in medium with wheat bran. K II was purified by precipitation with ammonium sulphate (20-80% saturation) and gel filtration on Biogel P-10. This enzyme is most active in hydrolysis of birchwood xylan at 50 degrees C and pH 5.5. Xylanase K II has an(More)
beta-glucosidase (EC 3.2.1.21) was isolated from the culture filtrate of Aspergillus niger IBT-90. The crude extracellular enzyme preparation was fractionated by six step purification procedure, (NH4)2SO4 precipitation, gel filtration on Bio-Gel P-10 and P-100, an ion-exchange chromatography on DEAE Bio-Gel A, yielding beta-glucosidase with an isoelectric(More)
Gordonia alkanivorans S7 is an efficient degrader of fuel oil hydrocarbons that can simultaneously utilize oxygen and nitrate as electron acceptors. The respiratory nitrate reductase (Nar) from this organism has been isolated using ion exchange chromatography and gel filtration, and then preliminarily characterized. PAGE, SDS-PAGE and gel filtration(More)
The thiol-dependent serine proteinase (inhibited by DFP, PMSF, pCMB and iodoacetate) was isolated from the whole krill specimens and from the content of the krill digestive tract. The enzyme was purified to homogeneity using a seven-step procedure. Its specific activity with denatured haemoglobin as a substrate was about 6.0 unit/mg. The molecular weight of(More)
INTRODUCTION The aim of the study was to evaluate two methods of macroprolactin (MaPRL) detection - precipitation with polyethylene glycol (PEG) and ultrafiltration and to compare these techniques with "gold standard" - gel filtration chromatography (GFC). MATERIAL AND METHODS The study was conducted on 245 patients - 45 with organic and 200 with(More)