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Rose rosette was first described in the early 1940s and it has emerged as one of the most devastating diseases of roses. Although it has been 70 years since the disease description, the rosette agent is yet to be characterized. In this communication, we identify and characterize the putative causal agent of the disease, a negative-sense RNA virus and new(More)
The complete nucleotide sequence of a novel member of the genus Crinivirus (family Closteroviridae), isolated from blackberry and tentatively named Blackberry yellow vein associated virus, was determined. The virus possesses a bipartite genome. RNA 1 is 7,801 nucleotides in length and papain-like protease, methyltransferase, RNA helicase and RNA-dependent(More)
In our effort to identify the causal agent(s) of strawberry pallidosis we found a single pallidosis positive plant that did not give any amplicons after RT-PCR using primer sets representing multiple regions of Strawberry pallidosis associated virus (SPaV) genome and failed to react with antibodies directed against the recombinant coat protein (CP) of SPaV.(More)
Flexuous filaments, resembling flexivirus virions, were observed in partially purified blackberry preparations showing mild virus-like symptoms. Further tests revealed the presence of a novel betaflexivirus that is phylogenetically related to foveaviruses. The putative virus-encoded proteins shared limited similarity with orthologs of known members of the(More)
The sequence of Lettuce chlorosis virus (LCV) (genus Crinivirus) was determined and found to contain unique open reading frames (ORFs) and ORFs similar to those of other criniviruses, as well as 3' non-coding regions that shared a high degree of identity. Northern blot analysis of RNA extracted from LCV-infected plants identified subgenomic RNAs(More)
Members of the family Closteroviridae have emerged as a major problem in agricultural crops in the past two decades. Diodia vein chlorosis virus (DVCV) is an understudied whitefly-transmitted closterovirus. Given the presence of the primary host for the virus in major agricultural production areas in the United States, we characterized the virus at the(More)
The complete nucleotide sequence of tomato infectious chlorosis virus (TICV) was determined and compared with those of other members of the genus Crinivirus. RNA 1 is 8,271 nucleotides long with three open reading frames and encodes proteins involved in replication. RNA 2 is 7,913 nucleotides long and encodes eight proteins common within the genus(More)
ABSTRACT A serious disease was observed in black raspberry (Rubus occidentalis) in Oregon in the last decade. Plants showing mosaic symptoms declined rapidly and, in many cases, died after several years. Double-stranded RNA extraction from symptomatic black raspberry revealed the presence of two high molecular weight bands which were cloned and sequenced.(More)
Blackberry yellow vein disease causes devastating losses on blackberry in the south and southeastern United States. Blackberry yellow vein associated virus (BYVaV) was identified as the putative causal agent of the disease but the identification of latent infections of BYVaV led to the investigation of additional agents being involved in symptomatology. A(More)
Raspberry mosaic is one of the most important viral diseases of raspberry. Four virus and virus-like agents, two of which are poorly characterized, have been implicated in the disease complex based on symptom development in Rubus indicators. Three novel viruses were identified in a red raspberry plant that caused typical raspberry mosaic symptoms when(More)