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In our effort to identify the causal agent(s) of strawberry pallidosis we found a single pallidosis positive plant that did not give any amplicons after RT-PCR using primer sets representing multiple regions of Strawberry pallidosis associated virus (SPaV) genome and failed to react with antibodies directed against the recombinant coat protein (CP) of SPaV.(More)
Blueberry and cranberry are fruit crops native to North America and they are well known for containing bioactive compounds that can benefit human health. Cultivation is expanding within North America and other parts of the world raising concern regarding distribution of existing viruses as well as the appearance of new viruses. Many of the known viruses of(More)
The genus Crinivirus includes the whitefly-transmitted members of the family Closteroviridae. Whitefly-transmitted viruses have emerged as a major problem for world agriculture and are responsible for diseases that lead to losses measured in the billions of dollars annually. Criniviruses emerged as a major agricultural threat at the end of the twentieth(More)
Flexuous filaments, resembling flexivirus virions, were observed in partially purified blackberry preparations showing mild virus-like symptoms. Further tests revealed the presence of a novel betaflexivirus that is phylogenetically related to foveaviruses. The putative virus-encoded proteins shared limited similarity with orthologs of known members of the(More)
The complete nucleotide sequence of Strawberry pallidosis associated virus (SPaV), a newly identified member of the genus Crinivirus, family Closteroviridae has been determined. RNA 1 is 8067 nucleotides long and encodes at least three open reading frames (ORFs). The first ORF (ORF 1a) specifies a multifunctional protein that has papain-like proteinase,(More)
Black locust (Robinia pseudoacacia L.) is an ornamental legume tree susceptible to several viruses. Virus diseases of black locust often go unrecognized making infected trees a prime inoculum source, not only for legumes, but also other plant species. In this communication we report, for the first time, two viruses, Papaya ringspot virus and Watermelon(More)
A novel virus was discovered in a blackcurrant accession (Ribes nigrum L.) at the USDA genebank in Oregon, USA. The genome consists of two positive-sense, single-stranded RNAs with the first encoding a 197 kDa multifunctional protein with methyl transferase, helicase and RNA-dependent RNA polymerase enzymatic motifs. The second molecule encodes two putative(More)
Blackberry chlorotic ringspot virus is a subgroup 1 ilarvirus, detected in several rosaceous hosts exhibiting disease symptoms in Europe and the United States. The population structure of the virus was studied using isolates collected from wild and cultivated plants from six states in the United States. The results suggest a homogeneous virus population in(More)
Fragaria chiloensis latent virus (FClLV), a member of the genus Ilarvirus was first identified in the early 1990s. Double-stranded RNA was extracted from FClLV infected plants and cloned. The complete nucleotide sequence of the virus has been elucidated. RNA 1 encodes a protein with methyltransferase and helicase enzymatic motifs while RNA 2 encodes the(More)
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