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An enhanced system for unnatural amino acid mutagenesis in E. coli.
We report a new vector, pEVOL, for the incorporation of unnatural amino acids into proteins in Escherichia coli using evolved Methanocaldococcus jannaschii aminoacyl-tRNA synthetase(s)Expand
A regenerative approach to the treatment of multiple sclerosis
Progressive phases of multiple sclerosis are associated with inhibited differentiation of the progenitor cell population that generates the mature oligodendrocytes required for remyelination andExpand
Identification of a small molecule with activity against drug-resistant and persistent tuberculosis
Significance The global problem of TB has worsened in recent years with the emergence of drug-resistant organisms, and new drugs are clearly needed. In a cell-based high-throughput screen, a smallExpand
An evolved aminoacyl-tRNA synthetase with atypical polysubstrate specificity.
We have employed a rapid fluorescence-based screen to assess the polyspecificity of several aminoacyl-tRNA synthetases (aaRSs) against an array of unnatural amino acids. We discovered that aExpand
Somatic hypermutation maintains antibody thermodynamic stability during affinity maturation
Somatic hypermutation and clonal selection lead to B cells expressing high-affinity antibodies. Here we show that somatic mutations not only play a critical role in antigen binding, they also affectExpand
Design of Switchable Chimeric Antigen Receptor T Cells Targeting Breast Cancer.
Chimeric antigen receptor T (CAR-T) cells have demonstrated promising results against hematological malignancies, but have encountered significant challenges in translation to solid tumors. ToExpand
Reshaping Antibody Diversity
Some species mount a robust antibody response despite having limited genome-encoded combinatorial diversity potential. Cows are unusual in having exceptionally long CDR H3 loops and few V regions,Expand
Evolution of cyclic peptide protease inhibitors
We report a bacterial system for the evolution of cyclic peptides that makes use of an expanded set of amino acid building blocks. Orthogonal aminoacyl-tRNA synthetase/tRNACUA pairs, together with aExpand
A metabolite-derived protein modification integrates glycolysis with KEAP1-NRF2 signaling
Mechanisms that integrate the metabolic state of a cell with regulatory pathways are necessary to maintain cellular homeostasis. Endogenous, intrinsically reactive metabolites can form functional,Expand
Expanding the genetic repertoire of the methylotrophic yeast Pichia pastoris.
To increase the utility of protein mutagenesis with unnatural amino acids, a recombinant expression system in the methylotrophic yeast Pichia pastoris was developed. Aminoacyl-tRNAExpand