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We have cloned the cDNA encoding the rat tissue inhibitor of metalloproteinase 3 (TIMP-3) by a PCR cloning method. Sequence analysis reveals an open reading frame containing 211 amino acids that show 99% identity to mouse TIMP-3 and 95% identity to human TIMP-3, respectively. High-level expression of TIMP-3 was detected in rat kidney, lungs and heart.
In an effort to identify novel genes relevant to tumor angiogenesis, we compared the genes expressed in a matched pair composed of vascularized breast tumor and its adjacent normal tissue obtained from the same cancer patient. Using differential display, we identified a cDNA fragment that was reproducibly upregulated in vascularized breast tumor.(More)
Cartilage is an avascular and relatively tumor-resistant tissue. Work from a number of laboratories, including our own, has demonstrated that cartilage is an enriched source of endogenous inhibitors of angiogenesis. In the course of a study designed to identify novel cartilage-derived inhibitors of new capillary growth, we have purified an inhibitory(More)
One of the most promising and exciting anti-cancer strategies is one that is based on the inhibition of angiogenesis. Unlike conventional therapeutic approaches, anti-angiogenic therapy holds the unique promise of treating the disease in the absence of cytotoxicity or drug resistance. The link between angiogenesis and the progression of human breast cancer(More)
We have cloned and sequenced the cDNA encoding the rat tissue inhibitor of metalloproteinase 4 (TIMP-4). As a first step towards exploring the role of TIMP-4 in both the physiological and the pathological remodeling of extracellular matrix, we have extensively analyzed the mRNA expression of TIMP-4 in various rat tissues. The results reveal that the(More)
Using modified differential display, a gene fragment was identified as being over-expressed in human vascularized breast carcinoma when compare to its neighboring normal tissue. The differentially expressed pattern was confirmed by quantitative RT-PCR. Full-length cDNA was then cloned by both 3'-end RACE and 5'-end RACE. Analysis of the full-length cDNA of(More)
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