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Homogenous yeast (Endomycopsis sp. 20-9) glucoamylase was isolated from cultural medium. The homogeneity of the enzyme preparation is demonstrated by means of polyacrylamide gel disc electrophoresis, isofocusing and ultracentrifugation. Amino acid composition, molecular weight (53 000), sedimentation constant (4.3S) and isoelectric point (pI 3.80-3.82) of… (More)
The effect of amino acids (glutamic, aspartic, beta-alanine, phenylalanine, methionine) on the glucoamylase biosynthesis by Endomycopsis species 20-9 was studied. Monoaminodicarboxylic acids produced a stimulating effect on the glucoamylase synthesis. Their addition at optimal concentrations of 0.2 to 0.4% increased by 54--69% glucoamylase activity of… (More)
The effects of low (1 . 10(-4) M) and high (1 . 10(-3) M) concentrations of n-propanol, isobutanol and isoamylols on the kinetic behaviour of "biosynthetic" L-threonine dehydratase from brewer's yeast S. carlsbergensis 776 were studied. It was concluded that these alcohols control the activity of the first enzyme of the L-threonine biosynthetic pathway.
Two chromatographic processes for purification of cyclodextringlucanotransferase (CGTase) from Bacillus sp. 1070 was carried out. The enzyme has been purified into 9.5 times on Butyl-Toyopearl and followed immobilized metal ion chromatography on Cu(II)-Iminodiacetic (IDA)-agarose. By the application of second purification scheme (chromatography on… (More)
The production of celulases by a soil yeast belonging to the genus Trichosporon and by microscopic fungi of various systematic groups was studied. The yeast synthesized relatively small amounts of cellulases in contrast to microscopic fungi. The active culture producing cellulases was the fungus Trichoderma longibrachiatum 7-26.
Dynamics of formation of by-products was studied with the following yeast races: Sacch. cerevisiae, strain Odesskaya-14 (baker's yeast); Sacch. vini, strain Prikumskaya 80/9 (wine yeast). The yeast cultures were found to be very similar by the rate of biomass accumulation, ethanol production, and the fractional composition of nitrogen compounds. The… (More)