Reprogramming of human somatic cells to pluripotency with defined factors
The data demonstrate that defined factors can reprogramme human cells to pluripotency, and establish a method whereby patient-specific cells might be established in culture.
Disease-Specific Induced Pluripotent Stem Cells
Large intergenic non-coding RNA-RoR modulates reprogramming of human induced pluripotent stem cells
It is found that one such lincRNA (lincRNA-RoR) modulates reprogramming, thus providing a first demonstration for critical functions of lincRNAs in the derivation of pluripotent stem cells.
Corrigendum: Targeted and genome-scale strategies reveal gene-body methylation signatures in human cells
Targeted and genome-scale strategies reveal gene-body methylation signatures in human cells and provide new insights into the regulation of methylation in cells.
Differential methylation of tissue- and cancer-specific CpG island shores distinguishes human induced pluripotent stem cells, embryonic stem cells and fibroblasts
Substantial hypermethylation and hypomethylation of cytosine-phosphate-guanine island shores in nine human iPS cell lines as compared to their parental fibroblasts are found, suggesting two mechanisms for epigenetic reprogramming in iPS cells and cancer.
Live cell imaging distinguishes bona fide human iPS cells from partially reprogrammed cells
Using serial live imaging of human fibroblasts undergoing reprogramming to identify distinct colony types that morphologically resemble embryonic stem cells yet differ in molecular phenotype and differentiation potential, it is determined that only one colony type represents true iPS cells, whereas the others represent reprograming intermediates.
Stage-specific signaling through TGFβ family members and WNT regulates patterning and pancreatic specification of human pluripotent stem cells
The temporal requirements for TGFβ family members and canonical WNT signaling are elucidated and it is shown that the duration of nodal/activin A signaling plays a pivotal role in establishing an appropriate definitive endoderm population for specification to the pancreatic lineage.
Mutant induced pluripotent stem cell lines recapitulate aspects of TDP-43 proteinopathies and reveal cell-specific vulnerability
- B. Bilican, A. Serio, S. Chandran
- BiologyProceedings of the National Academy of Sciences
- 26 March 2012
It is concluded that expression of physiological levels of TDP-43 in human neurons is sufficient to reveal a mutation-specific cell-autonomous phenotype and strongly supports this approach for the study of disease mechanisms and for drug screening.
A role for Lin28 in primordial germ cell development and germ cell malignancy
It is shown that Blimp1 (also called Prdm1), a let-7 target and a master regulator of PGC specification, can rescue the effect of Lin28 deficiency during PGC development, thereby establishing a mechanism of action for Lin28 during P GC specification.
Regulatory networks define phenotypic classes of human stem cell lines
A database of global gene expression profiles that enables the classification of cultured human stem cells in the context of a wide variety of pluripotent, multipotent and differentiated cell types is created and analysis of this database offers a new strategy for classifying stem cells.