In Doo Hwang

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The immunoscreening method was used to isolate cDNAs of 1323 bp (ClOCT1) and 1433 bp (ClOCT2) encoding two ornithine carbamoyltransferases (OCT, EC 2.1.3.3) from the cDNA expression library ofCanavalia lineata leaves constructed in a λZAP Express vector. ClOCT1 and ClOCT2 encode 359 and 369 amino acids, respectively. The N-terminals of deduced amino acid(More)
Maximum shoot induction from stem explants ofCanavalia lineata was obtained with an agar-solidified PC medium containing 10 μM benzylaminopurine and 1 μM naphthaleneacetic acid. Rooting of thesein vitro produced shoots was achieved with hormone-free PC medium. Canavanine was produced almost exclusively in the leaves and was not detected in the roots ofin(More)
The greening of callus was achieved by modulating the medium's growth regulator concentrations under continuous light. Canavalia lineata (L.) DC. calluses formed chlorophyll when they were exposed to continuous light in the presence of benzylaminopurine and indole-3-acetic acid. Canavanine and canaline were detected in the green callus. But only canaline(More)
Effect of spermine on the phytochelatins (PCs) in cadmium-treated roots ofCanavalia lineata seedlings was studied. With the treatment of spermine, total nonprotein thiol (SH) contents decreased by 55% in roots of Cd-treated plants. Glutathione (GSH) synthetase activity was inhibited by 36.8% in roots and cysteine synthase was also inhibited by 9.5% while(More)
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