Ilya Elashvili

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Null mutants of superoxide dismutase (SOD) in Saccharomyces cerevisiae are associated with a number of biochemical defects. In addition to being hypersensitive to oxygen toxicity, strains containing deletions in both the SOD1 (encoding Cu/Zn-SOD) and SOD2 (encoding Mn-SOD) genes are defective in sporulation, are associated with a high mutation rate, and are(More)
Remediation efforts for the oil spill from the Exxon Valdez tanker in Alaska have focused on the use of pressurized water at high temperature to remove oil from the beaches. We have tested a biological surfactant from Pseudomonas aeruginosa for its ability to remove oil from contaminated Alaskan gravel samples under various conditions, including(More)
Recombinant forms of the bacteriophage MS2 and its RNA-free (empty) MS2 capsid were analyzed in solution to determine if RNA content and/or the A (or maturation) protein play a role in the global arrangement of the virus protein shell. Analysis of the (coat) protein shell of recombinant versions of MS2 that lack the A protein revealed dramatic differences(More)
Small-angle neutron scattering (SANS) has been used to extend the structural characterization of the MS2 phage by examining its physical characteristics in solution. Specifically, the contrast variation technique was employed to determine the molecular weight of the individual components of the MS2 virion (protein shell and genomic RNA) and the spatial(More)
Wild-type Escherichia coli K-12 strain JA221 grows poorly on low concentrations (< or = 1 mM) of diisopropyl fluorophosphate and its hydrolysis product, diisopropyl phosphate (DIPP), as sole phosphorus sources. Spontaneous organophosphate utilization (OPU) mutants were isolated that efficiently utilized these alternate sources of phosphate. A genomic(More)
0969-80 doi:10.1 fax: +1 E-m Prese P.O. Bo Prese sion, De man Rd Prese Gaither in Quantification of RNA in bacteriophage MS2-like viruses solution by small-angle X-ray scattering Deborah A. Kuzmanovic, Ilya Elashvili, Charles Wick, Catherine O’Connell, Susan Krueger Biotechnology Division, NIST, 100 Bureau Drive, Stop 8311, Gaithersburg, MD 20899-8311, USA(More)
ABSTRACT In this study, we demonstrate the effect of sample matrix composition of MS2 virus on its characterization by ESI-MS and IVDS. MS2 samples grown and purified using various techniques showed different responses on ESI-MS than that on IVDS. The LC-MS of the specific biomarker of MS2 bacteriophage from an infected Escherichia coli sample was(More)
We have developed a rapid and highly reliable analytical methodology for current and prospective nerve agents and their degradation products. The approach is to augment existing analytical methods with a specific enzymatic transformation step. It utilizes a novel bacterial phosphonate ester hydrolase enzyme (PEH), which degrades the agents’ alkali(More)
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