Ikuko Nakajima

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An efficient method for the generation of adventitious shoots from the cotyledons of Japanese pear and related species was developed. Cotyledons from seeds of the Japanese pear “Okusankichi” and the Asian pea pear “Hokushimamenashi” were used to determine the optimum concentrations of phytohormones in the medium. The rates of generation of adventitious(More)
Genetic transformation was successfully established producing both transformed adventitious shoots and calli in Japanese pear (Pyrus pyrifolia Nakai) by using cotyledons as explants. Cotyledons of five cultivars were co-cultivated with Agrobacterium tumefaciens strain LBA4404 carrying the pBIN19-sgfp, which contained a green fluorescent protein gene and the(More)
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