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Glutamate decarboxylase, which is associated with a glutamate-dependent acid-resistance mechanism, was purified from Lactococcus lactis subsp. lactis by a three-step procedure. The specific activity was increased about 114-fold with a yield of 16%. The N-terminal amino acid sequence of the enzyme was determined. The gene encoding this enzyme was cloned in(More)
We studied the spatial distribution of the pattern-evoked potentials on the face with a simultaneous 16-channel recording system. The results showed that the responses had their first positive component at the peak latency of 52.0 +/- 1.9 (S.E.) msec. The maximum potential value within the equipotential maps was in the region around the stimulated eye in(More)
An efficient method for the generation of adventitious shoots from the cotyledons of Japanese pear and related species was developed. Cotyledons from seeds of the Japanese pear “Okusankichi” and the Asian pea pear “Hokushimamenashi” were used to determine the optimum concentrations of phytohormones in the medium. The rates of generation of adventitious(More)
Genetic transformation was successfully established producing both transformed adventitious shoots and calli in Japanese pear (Pyrus pyrifolia Nakai) by using cotyledons as explants. Cotyledons of five cultivars were co-cultivated with Agrobacterium tumefaciens strain LBA4404 carrying the pBIN19-sgfp, which contained a green fluorescent protein gene and the(More)
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