Igor V Kubasov

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Nicotine in concentration 100 nM which corresponds to concentration of nicotine circulating in tobacco smokes induced hyperpolarization by approximately 4 mV of muscle fibres of the rat isolated diaphragm, as well as an increase in amplitude and acceleration of action potentials. Similar hyperpolarization was induced by nicotine and acetylcholine in the rat(More)
Two types of muscle fibre action potentials (APs) were recorded using narrow-tipped extracellular pipettes in isolated sartorius muscles of frog, Rana temporaria. The waveform of type 1 responses (T1 AP, 75% of recordings) was biphasic, 'positive–negative.' The type 2 signals were tri-phasic, 'positive–negative–positive' (T2 AP, 18%). The type of AP was(More)
The effect of insulin on contractility of directly stimulated skeletal muscles was studied in experiments in isolated preparations of rat fast, extensor digitorum longus (m. EDL), slow, soleus (m. SOL) and mixed, diaphragm muscles. In addition (diaphragm only) characteristics of extracellularly recorded muscle fiber action potentials (APs) were evaluated(More)
After three days of hind limb unloading, the depolarization of muscle fibers from -71.0 +/- 0.5 mV to -66.8 +/- 0.7 mV as well as a decrease in muscle excitability and a trend to fatigue acceleration were observed. After hind limb unloading, the electrogenic contribution of the ouabain-sensitive alpha2 isoform of Na,K-ATPase, tested as depolarization due to(More)
The last contractile responses (LCRN), where N is the number of individual contractile responses within tetanus, were separated from the integral tetanic responses of fast, m. Extensor digitorum longus (m. EDL), and slow, m. Soleus, rat muscles using a computer-graphic technique. The average amplitude of LCR5 in m. Soleus at a 20 Hz stimulation rate(More)
Using a computer graphics approach, the last contractile responses (LCRN, where N is a number of elementary contractile responses in tetanus) were separated from integral tetanic responses of rat fast muscles, m. Eхtensor digitorum longus (m. EDL), and slow muscles, m. Soleus, evoked by trains of 5, 10 and 50 stimuli. In m. Soleus, at a stimulation(More)
Effects of Na+,K(+)-ATPase inhibitor: marinobufagenin, on contractile and electric characteristics of isolated rat diaphragm were studied for the first time. Marinobufagenin induced dose-dependent (EC50 = 0.3 +/- 0.1 nM) increase in the contraction force (positive inotropic effect). At 1-2 nM, it slowed down the fatigue induced by continuous direct(More)