Ibrahim M. Seraj

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The present study demonstrated the presence of HPV gene sequences in Percoll-washed sperm cells using polymerase chain reaction primers targeting smaller gene regions. Up to 64% of the sperm specimens were shown to contain gene sequences indicative of the presence of HPV. Human papillomavirus type 16 was detected about twice as often as HPV type 18. The(More)
Ovarian carcinoma is one of the frequent causes of death from malignancies in the United States. A report excited the scientific community when human papillomavirus were identified in advanced epithelial ovarian carcinoma tissues in 10 of 12 patients. A few studies also identified HPV DNA in ovarian carcinoma tissues. However, several researchers employing(More)
The objective of the study was to determine if embryos at the blastocyst stage differentially took up exogenous human papillomavirus (HPV) DNA derived from the different HPV types and to determine whether the capture process was active or passive. In vivo fertilized mouse embryos were cultured to the blastocyst stage in vitro. The mouse blastocysts were(More)
2. Hillier SG, Parsons JH, Margara RM, et al.: Serum estradiol and preovulatory follicular development before in-vitro fertilization. J Endocrinol 1984; I 01 : 113 3. Barak Y, Lessing JB, Kogosowski A, et al.: The development of an efficient ambulatory in vitro fertilization (IVF) and embryo transfer (ET) programme using ultrasonically guided oocyte(More)
The ability to predict myometrial invasion by endometrial adenocarcinoma is useful for preoperative treatment purposes. The presence of stromal invasion is one possible method of predicting myometrial invasion, but criteria for the presence of stromal invasion have been lacking. Recently, criteria for its detection were proposed. We evaluated the validity(More)
A retrospective analysis of 24 patients with early invasive squamous carcinoma was performed. No nodal metastases were noted in these patients. Based on a review of the literature, no absolute definition of microinvasive carcinoma could be formulated, but a treatment outline has been formulated based on depth of invasion for Stage I lesions.
Recently, it was demonstrated that sperm cells could be used to carry foreign DNA or exogenous DNA fragments through an artificial reproductive tract and transfer the DNA to mouse blastocysts at the other end of the tract (1). Using radiolabeling methods, the DNA fragments have been reported to be localized at the postacrosomal and equatorial regions of the(More)
Mycoplasmas are tiny polymorphic prokaryotic organisms (0.2-0.3 microm) that lack a cell wall and reside ubiquitously at the cell membrane or internalized into the cell. The organisms have been implicated in many diseases including functioning as cofactors catalyzing the HIV disease state. The oncogenic potential of mycoplasmas was only recently realized(More)
OBJECTIVE To determine the prevalence of mycoplasmas in washed sperm and to compare the penetration of zona-free hamster oocytes by sperm with and without mycoplasmas. DESIGN Prospective comparative study. SETTING Clinical and academic research environment. PATIENT(S) Semen from 34 male patients. INTERVENTION(S) Specimens were divided, Percoll(More)
Clinically, it is important to detect mycoplasmas because these organisms have been implicated in gastric and ovarian cancer, pneumonia, postabortal fever, pelvic inflammatory disease, pyelonephritis, endometritis, urethritis, perinatal mortality, arthritis, spontaneous abortion, infertility and interference with sperm development and they act as cofactors(More)