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The RAG proteins and V(D)J recombination: complexes, ends, and transposition.
V(D)J recombination proceeds through a series of protein:DNA complexes mediated in part by the RAG1 and RAG2 proteins. These proteins are responsible for sequence-specific DNA recognition and DNAExpand
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Identification of two catalytic residues in RAG1 that define a single active site within the RAG1/RAG2 protein complex.
During V(D)J recombination, the RAG1 and RAG2 proteins cooperate to catalyze a series of DNA bond breakage and strand transfer reactions. The structure, location, and number of active sites involvedExpand
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The Metallo-β-Lactamase/β-CASP Domain of Artemis Constitutes the Catalytic Core for V(D)J Recombination
The V(D)J recombination/DNA repair factor Artemis belongs to the metallo-β-lactamase (β-Lact) superfamily of enzymes. Three regions can be defined within the Artemis protein sequence: (a) the β-LactExpand
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DNA-Rag Protein Interactions in the Control of Selective D Gene Utilization in the TCRβ Locus1
Ordered assembly of Ag receptor genes by VDJ recombination is a key determinant of successful lymphocyte differentiation and function. Control of gene rearrangement has been traditionally viewed as aExpand
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Defect in rearrangement of the most 5' TCR-J alpha following targeted deletion of T early alpha (TEA): implications for TCR alpha locus accessibility.
To address the role of the TEA germline transcription, which initiates upstream of the TCR-J alpha S, in the regulation of TCR-J alpha locus accessibility, we created a mouse in which this region hasExpand
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Defect in Rearrangement of the Most 5′ TCR–Jα Following Targeted Deletion of T Early α (TEA): Implications for TCR α Locus Accessibility
Abstract To address the role of the TEA germline transcription, which initiates upstream of the TCR–Jαs, in the regulation of TCR–Jα locus accessibility, we created a mouse in which this region hasExpand
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Detection of RAG Protein-V(D)J Recombination Signal Interactions Near the Site of DNA Cleavage by UV Cross-Linking
ABSTRACT V(D)J recombination is initiated by double-strand cleavage at recombination signal sequences (RSSs). DNA cleavage is mediated by the RAG1 and RAG2 proteins. Recent experiments describing RAGExpand
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RORγT, a thymus‐specific isoform of the orphan nuclear receptor RORγ / TOR, is up‐regulated by signaling through the pre‐T cell receptor and binds to the TEA promoter
TEA (T early alpha) is a genetic element located upstream of the TCR‐Jα cluster. Thymocytes from mice carrying a targeted deletion of TEA do not rearrange their TCRα locus on a window spanning theExpand
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A dimer of the lymphoid protein RAG1 recognizes the recombination signal sequence and the complex stably incorporates the high mobility group protein HMG2.
RAG1 and RAG2 are the two lymphoid-specific proteins required for the cleavage of DNA sequences known as the recombination signal sequences (RSSs) flanking V, D or J regions of the antigen-bindingExpand
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