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A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V.
In the early stages of apoptosis changes occur at the cell surface, which until now have remained difficult to recognize. One of these plasma membrane alterations is the translocation ofExpand
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Electrospinning of collagen and elastin for tissue engineering applications.
Meshes of collagen and/or elastin were successfully prepared by means of electrospinning from aqueous solutions. Flow rate, applied electric field, collecting distance and composition of the startingExpand
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Flow cytometry of apoptotic cell death.
The term apoptosis or programmed cell death defines a genetically encoded cell death program, which is morphologically and biochemically distinct from necrosis or accidental cell death. TheExpand
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BBB ON CHIP: microfluidic platform to mechanically and biochemically modulate blood-brain barrier function
The blood-brain barrier (BBB) is a unique feature of the human body, preserving brain homeostasis and preventing toxic substances to enter the brain. However, in various neurodegenerative diseases,Expand
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Measuring apoptosis in human spermatozoa: a biological assay for semen quality?
OBJECTIVE [1] To determine whether apoptosis can be measured in ejaculated spermatozoa by flow cytometry using the Annexin V assay, which measures expression of phosphatidylserine on the outerExpand
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Macrophage migration inhibitory factor and hypothalamo-pituitary-adrenal function during critical illness.
In patients with septic shock (n = 32), multitrauma (n = 8), and hospitalized matched controls (n = 41), we serially measured serum macrophage inhibitory factor (MIF), cortisol, plasma ACTH, tumorExpand
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Patterns of corticosteroid-binding globulin and the free cortisol index during septic shock and multitrauma
Abstract.Objective: To study the time course of corticosteroid binding-globulin (CBG) level and the free cortisol index (FCI) in comparison with total cortisol and ACTH concentrations during acuteExpand
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Differential effects of progestogens on breast cancer cell lines.
Our in vitro results indicate that not all progestogens act equally on breast cancer cells. Some progestogens (medroxyprogesterone acetate (MPA), norethisterone acetate (NETA) and dienogest) alone orExpand
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High-yield cell ordering and deterministic cell-in-droplet encapsulation using Dean flow in a curved microchannel.
In this article high-yield (77%) and high-speed (2700 cells s(-1)) single cell droplet encapsulation is described using a Dean-coupled inertial ordering of cells in a simple curved continuousExpand
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Insulin-like growth factor (IGF)-I and IGF binding protein-3 concentrations in fluid from human stimulated follicles.
Insulin-like growth factor-I (IGF-I) and IGF binding protein-3 (IGFBP-3) play an important role in regulating follicle growth and maturation. We have evaluated whether responsiveness toExpand
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