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The SRA protein Np95 mediates epigenetic inheritance by recruiting Dnmt1 to methylated DNA
It is shown that localization of mouse Np95 to replicating heterochromatin is dependent on the presence of hemi-methylated DNA and the link between Np 95 and Dnmt1 establishes key steps of the mechanism for epigenetic inheritance of DNA methylation. Expand
DNMT3L Stimulates the DNA Methylation Activity of Dnmt3a and Dnmt3b through a Direct Interaction*
In mammals, the resetting of DNA methylation patterns in early embryos and germ cells is crucial for development. Two DNA methyltransferases, Dnmt3a and Dnmt3b, are responsible for the creation ofExpand
Array-based genomic resequencing of human leukemia
The data have shown a unique profile of gene mutations in human leukemia, and a somatic change responsible for an Arg-to-His substitution at amino-acid position 882 of DNA methyltransferase 3A, which resulted in a loss ofDNA methylation activity of >50%. Expand
Co-expression of de novo DNA methyltransferases Dnmt3a2 and Dnmt3L in gonocytes of mouse embryos.
The results strongly suggest that DnMT3a2 and Dnmt3L are responsible for the global DNA methylation in mouse male germ cells. Expand
Stage- and cell-specific expression of Dnmt3a and Dnmt3b during embryogenesis
It is demonstrated that the DnMT3a and Dnmt3b proteins are expressed at different stages of embryogenesis, which may contribute to their distinct functions during the embryogenesis. Expand
Distinct DNA methylation activity of Dnmt3a and Dnmt3b towards naked and nucleosomal DNA.
It is proposed that the preferential DNA methylation activity of Dnmt3a towards the naked part of nucleosomes reconstituted from recombinant histones and DNAs and the significant methylationActivity of DNmt3b towards the nucleosome core region contribute to their distinct methylation of genomic DNA in vivo. Expand
Enzymatic properties of de novo-type mouse DNA (cytosine-5) methyltransferases.
P purified GST-fused recombinant mouse Dnmt3a and three isoforms of mouse DNmt3b to near homogeneity showed similar activity toward poly(dG-dC)-poly(dD-DG-DC) for measuring de novo methylation activity, and toward poly (dI-dDC)- Poly(dI)-dC for measuring total activity, indicating that the enzymes are de noovo-type DNA methyltransferases. Expand
Structural insight into maintenance methylation by mouse DNA methyltransferase 1 (Dnmt1)
The crystal structure of the large fragment of mouse Dnmt1 and its complexes with cofactor S-adenosyl-L-methionine and its product S- adenosyl -L-homocystein is reported, suggesting that maintenance methylation is a multistep process accompanied by structural changes. Expand
Processive Methylation of Hemimethylated CpG Sites by Mouse Dnmt1 DNA Methyltransferase*
It is revealed that Dnmt1 methylates hemimethylated CpG sites on one strand of double-stranded DNA during a single processive run, suggesting a faithful and efficient maintenance of methylation patterns in the mammalian genome. Expand
Maintenance-type DNA methyltransferase is highly expressed in post-mitotic neurons and localized in the cytoplasmic compartment.
The findings that the Dnmt1 transcript in the brain utilized the somatic-type exon and that the apparent size of the DNmt1 protein in the cytoplasm was identical to that in proliferating culture cells indicate that the cy toplasmic DnMT1 in neurons was of the somatics-type. Expand