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Quaternary ligand binding to aromatic residues in the active-site gorge of acetylcholinesterase.
TLDR
The structural and chemical data show the important role of aromatic groups as binding sites for quaternary ligands in Torpedo acetylcholinesterase, and provide complementary evidence assigning Trp-84 and Phe-330 to the "anionic" subsite of the active site andtrp-279 to the 'peripheral' anionic site.
Quaternary ligand binding to aromatic residues in the active-site gorge of acetylcholinesterase.
TLDR
The structural and chemical data show the important role of aromatic groups as binding sites for quaternary ligands in Torpedo acetylcholinesterase, and provide complementary evidence assigning Trp-84 and Phe-330 to the "anionic" subsite of the active site andtrp-279 to the 'peripheral' anionic site.
Structure and mutational analysis of Rab GDP-dissociation inhibitor
The crystal structure of the bovine α-isoform of Rab GDP-dissociation inhibitor (GDI), which functions in vesicle-membrane transport to recycle and regulate Rab GTPases, has been determined to a
Presence of the siderophores pyoverdine and pyochelin in the extracellular medium reduces toxic metal accumulation in Pseudomonas aeruginosa and increases bacterial metal tolerance.
TLDR
Measurement by Inductively Coupled Plasma-Atomic Emission Spectrometry of the concentration of metals present in bacteria incubated with metals in the presence or absence of PVD or PCH indicated that both siderophores were able to sequester metals from the extracellular medium of the bacteria, decreasing metal diffusion into the bacteria.
Pyoverdine biosynthesis and secretion in Pseudomonas aeruginosa: implications for metal homeostasis.
TLDR
The cellular localization of pyoverdine precursors was recently shown to be consistent with their biosynthetic enzymes, and a recently identified ATP-dependent efflux pump, PvdRT-OpmQ, is considered, which contributes to the understanding of the role of py overdine in iron and metal homeostasis in fluorescent Pseudomonads.
New roles for bacterial siderophores in metal transport and tolerance.
TLDR
This article considers this new concept that siderophores play a role in protecting bacteria against metal toxicity and discusses the possible contribution of these chelators to the transport of biological relevant metals in addition to iron.
New insights into the metal specificity of the Pseudomonas aeruginosa pyoverdine-iron uptake pathway.
TLDR
The spectral properties of PvdI are used to show that in addition to Fe(3+), this siderophore also chelates, but with lower efficiencies, all the 16 metals used in the authors' screening, which allow a better understanding of the behaviour of the PvDI uptake pathway in the presence of metals other than iron.
Synthesis of the siderophore pyoverdine in Pseudomonas aeruginosa involves a periplasmic maturation
TLDR
Using the fluorescent properties of PVDI, the pyoverdine produced by Pseudomonas aeruginosa PAO1, it was able to show that PVD i was not present in the cytoplasm of the bacteria, but large amounts of a fluorescent PVDi precursor PVD Ip were stored in the periplasm.
Cell–cell contacts confine public goods diffusion inside Pseudomonas aeruginosa clonal microcolonies
TLDR
In Pseudomonas aeruginosa microcolonies growing on solid substrate, it is found that the concentration of pyoverdine, a secreted iron chelator, is heterogeneous, with a maximum at the center of the colony, and quantitatively explain the formation of this gradient by local exchange between contacting cells rather than by global diffusion of py overdine.
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