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Quantifying colocalization by correlation: The Pearson correlation coefficient is superior to the Mander's overlap coefficient
The Pearson correlation coefficient (PCC) and the Mander's overlap coefficient (MOC) are used to quantify the degree of colocalization between fluorophores. The MOC was introduced to overcome… Expand
Detergent-resistant membranes and the protein composition of lipid rafts
The plasma membrane of eukaryotic cells contains lipid rafts with protein and lipid compositions differing from the bulk plasma membrane. Several recent proteomic studies have addressed the… Expand
Cholesterol homeostasis in T cells. Methyl-beta-cyclodextrin treatment results in equal loss of cholesterol from Triton X-100 soluble and insoluble fractions.
Methyl-beta-cyclodextrin (MBCD) is frequently used to acutely deplete cells of cholesterol. A widespread assumption is that MBCD preferentially targets cholesterol in lipid rafts and that sensitivity… Expand
Plasma membrane topography and interpretation of single-particle tracks
To the Editor: Many contemporary models of the plasma membrane are based on single-particle tracking (SPT) by light microscopy on live cells. Whereas the analysis of single-particle tracks typically… Expand
Cholesterol depletion using methyl-β-cyclodextrin.
Cholesterol is an essential component of mammalian cells. It is the major lipid constituent of the plasma membrane and is also abundant in most other organelle membranes. In the plasma membrane… Expand
Replicate‐based noise corrected correlation for accurate measurements of colocalization
It is widely recognized that the accuracy of colocalization measurements is dependent upon the quality of the source images. We demonstrate that, as the image quality increases, the measured… Expand
Limited cholesterol depletion causes aggregation of plasma membrane lipid rafts inducing T cell activation.
- Saleemulla Mahammad, Jelena Dinić, J. Adler, I. Parmryd
- Biology, Medicine
- Biochimica et biophysica acta
- 1 June 2010
Acute cholesterol depletion is generally associated with decreased or abolished T cell signalling but it can also cause T cell activation. This anomaly has been addressed in Jurkat T cells using… Expand
Actin filaments attachment at the plasma membrane in live cells cause the formation of ordered lipid domains.
- Jelena Dinić, Parham Ashrafzadeh, I. Parmryd
- Chemistry, Medicine
- Biochimica et biophysica acta
- 1 March 2013
The relationship between ordered plasma membrane nanodomains, known as lipid rafts, and actin filaments is the focus of this study. Plasma membrane order was followed in live cells at 37°C using… Expand
Interleaflet Coupling, Pinning, and Leaflet Asymmetry—Major Players in Plasma Membrane Nanodomain Formation
The plasma membrane has a highly asymmetric distribution of lipids and contains dynamic nanodomains many of which are liquid entities surrounded by a second, slightly different, liquid environment.… Expand
Colocalization analysis in fluorescence microscopy.
The measurement of colocalization requires images of two fluorophores that are aligned, with no cross talk, and that the intensities remain within the response range of the microscope. Quantitation… Expand