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Recent advances in 2D and 3D in vitro systems using primary hepatocytes, alternative hepatocyte sources and non-parenchymal liver cells and their use in investigating mechanisms of hepatotoxicity,
This review encompasses the most important advances in liver functions and hepatotoxicity and analyzes which mechanisms can be studied in vitro and how closely hepatoma, stem cell and iPS cell–derived hepatocyte-like-cells resemble real hepatocytes. Expand
Cysteine 38 in p65/NF-κB Plays a Crucial Role in DNA Binding Inhibition by Sesquiterpene Lactones*
Contrary to previous reports, it is shown that parthenolide, like other SLs, inhibits NF-κB most probably by alkylating p65 at Cys38, and the amount of remaining IκB was too low to explain the observed NF-β degradation inhibition. Expand
The Anti-inflammatory Sesquiterpene Lactone Helenalin Inhibits the Transcription Factor NF-κB by Directly Targeting p65*
This sesquiterpene lactone helenalin is the first anti-inflammatory agent shown to exert its effect by directly modifying NF-κB, the central mediator of the human immune response. Expand
Inhibition of transcription factor NF-kappaB by sesquiterpene lactones: a proposed molecular mechanism of action.
A molecular mechanism of action is proposed, which is able to explain the p65 selectivity of the SLs and the observed correlation of high activity with alkylant bifunctionality. Expand
Anti-oxidant, anti-inflammatory and anti-allergic activities of luteolin.
In vivo and in vivo, luteolin reduced increased vascular permeability and was effective in animal models of inflammation after parenteral and oral application, indicating that it has the potential to protect from diseases associated with inflammatory processes such as cardiovascular disease. Expand
ON/OFF and Beyond - A Boolean Model of Apoptosis
A large-scale literature-based Boolean model of the central intrinsic and extrinsic apoptosis pathways as well as pathways connected with them is built and gives new insights into the complex interplay of pro- and antiapoptotic factors and can be easily expanded to other signaling pathways. Expand
Determination of the wound healing effect of Calendula extracts using the scratch assay with 3T3 fibroblasts.
The scratch assay in the present form can be used as a promising scientific approach and platform to differentiate between plant extracts known for their wound healing and their anti-inflammatory properties. Expand
Perspectives on sesquiterpene lactones in inflammation and cancer.
  • I. Merfort
  • Biology, Medicine
  • Current drug targets
  • 30 September 2011
Sesquiterpene lactones may possess therapeutic relevance as single components for the local treatment of inflammation, such as rheumatoid complaints and in cancer therapy, SLs may be favourable in dual therapy or in the inhibition of leukaemia cell growth. Expand
Role of cysteine residues of p65/NF-kappaB on the inhibition by the sesquiterpene lactone parthenolide and N-ethyl maleimide, and on its transactivating potential.
The mechanism of the sesquiterpene lactone-mediated inhibition was analyzed using different cell systems, and it was shown that in all the cell lines analyzed, SLs inhibited both NF-kappaB binding and the IkappaB-kinase, but that the former played a more preponderant role in the inhibition. Expand
Anti-inflammatory effects of glycyrol isolated from Glycyrrhiza uralensis in LPS-stimulated RAW264.7 macrophages.
Results indicate that glycyrol is an important anti-inflammatory constituent of Glycyrrhizae Radix, and that its anti- inflammatory effect is attributed to the inhibition I-kappaBalpha phosphorylation. Expand