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Journals and Conferences
The primary structure of bacteriophage lambda DNA has been searched for the presence of consensus CAP binding sites. Four putative CAP binding sites have been found on the lambda genome, indicating… (More)
The plasmid pNT6 permits selection of IS1-element insertions into the plasmid occurring with the frequency about 10%. The bacteriophage promoter pL cloned in pNT6 is a hot-spot region for IS1-element… (More)
A variety of approaches to determine the complete sequence of large DNA fragments is summarized. Main attention is paid to a transposon-based DNA sequencing strategy.
Cloning vèctor lambda gt-p MB9 has been used for cloning of DNA fragments of bacteriophage T5 produced by EcoR*I activity. One clone contains a DNA fragment of 2.2 Md which has been mapped at 67-71%… (More)
To study the mechanism of precise excision ofgypsy from genomic sites, the integrase domain ofgypsy pol was cloned and expressed inEscherichia coli. The endonuclease activity of recombinant integrase… (More)
The expression of oLpLN region of the plasmid pNT6 causes the high instability of the plasmid. Mutations in the promoter pL region and lesions in the structural part of the N gene result in the… (More)