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Serotyping of Serratia marcescens: evaluation of Le Minor's H-immobilization test and description of three new flagellar H antigens
The H-immobilization test of Le Minor for determining flagellar (H) antigens was evaluated and compared with tube and slide H-agglutination tests and three new provisional H antignes, designated H14, H15, and H16, are proposed.
Serotyping of Serratia marcescens: determination of serogroup (O) antigens and serological cross-reactions.
Transfection of Escherichia coli Spheroplasts I. General Facilitation of Double-Stranded Deoxyribonucleic Acid Infectivity by Protamine Sulfate
The addition of 25 μg of protamine sulfate per ml to lysozyme-ethylenediamine-tetraacetic acid spheroplasts of Escherichia coli stimulates transfection not only for T1 phage deoxyribonucleic acid…
Differences of two Borrelia burgdorferi strains in complement activation and serum resistance.
Selective activation of classical and alternative pathways of human complement by "promptly serum-sensitive" and "delayed serum-sensitive" strains of Serratia marcescens
Chelation of fresh human serum with 0.1 M MgCl2 plus 0.01 M ethylenediametetracetate failed to abrogate the bactericidal activity against "delayed serum-sensitive" strains of Serratia marcescens, whereas previously "promptly serum- sensitive" strain S. marces censorship was killed after an extended period of incubation.
Studies on the additive effect of polymyxin B and the bactericidal activity of human serum against Serratia marcescens.
The combination of therapeutically achievable concentrations of polymyxin B and fresh, but not heat-inactivated human serum was found to exert an accelerated, additive effect against 9 of 10 'delayed serum-sensitive' isolates of S. marcescens, an organism that is characterized by intrinsic resistance againstpolymyxins.
Detection of fibrinolytic and elastase activity among clinical isolates of Serratia marcescens.
Thirty-three of 35 clinical isolates of Serratia marcescens examined displayed fibrinolytic activity, whereas only 5 isolates elaborated a weakly active elastase. All strains liquef
Characterization of a nosocomially significant, multiple drug-resistant strain of Serratia marcescens.
A multiple drug-resistant strain of Serratia marcescens (bacteriocin type 18) was isolated from three clinical patients. The isolates were found to carry a conjugally nontransferable, nonmobilizeable…
Transfection of Escherichia coli Spheroplasts III. Facilitation of Transfection and Stabilization of Spheroplasts by Different Basic Polymers
The only compound which fully replaced protamine sulfate in facilitating transfection of Escherichia coli spheroplasts by phage DNAs was spermine; poly-l-lysine, poly-l-arginine, DEAE-dextran,…
Ultrastructural surface alterations of serratia marcescens after exposure to polymyxin B and/or fresh human serum.
The combination of polymyXin B and fresh human serum caused clearly discernible ultrastructural changes of the polymyxin and the fresh serum type, respectively.