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Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems
- I. H. Segel
- Chemistry, Biology
- 12 May 1975
Characterization of the cellulose-binding domain of the Clostridium cellulovorans cellulose-binding protein A
- M. Goldstein, M. Takagi, S. Hashida, O. Shoseyov, R. Doi, I. H. Segel
- BiologyJournal of Bacteriology
- 1 September 1993
This CBD is of particular interest because it is the first CBD from a completely sequenced nonenzymatic protein shown to be an independently functional domain and suggests that the CBD recognition site is larger than a simple cellobiose unit or more complex than a repeatingcellobiose moiety.
Purification and properties of glycogen phosphorylase from Escherichia coli.
ATP sulfurylase from higher plants : purification and preliminary kinetics studies on the cabbage leaf enzyme.
Initial velocity and product inhibition studies of the forward and reverse reactions point to an obligately ordered kinetic mechanism with MgATP adding before MoO(4) (2-) and MgPPi leaving before AMP + MoO (4)(2-) (or adenosine-5'-phosphosulfate [APS]).
Crystal structure of ATP sulfurylase from Penicillium chrysogenum: insights into the allosteric regulation of sulfate assimilation.
ATP sulfurylase from Penicillium chrysogenum is an allosterically regulated enzyme composed of six identical 63.7 kDa subunits (573 residues) and the structure presented here is believed to be the R state.
Adenosine 5'-phosphosulfate kinase from Penicillium chrysogenum. Purification and kinetic characterization.
ATP sulfurylase from trophosome tissue of Riftia pachyptila (hydrothermal vent tube worm).
Characterization of an ammonium transport system in filamentous fungi with methylammonium-14C as the substrate.
ATP sulfurylase from higher plants: kinetic and structural characterization of the chloroplast and cytosol enzymes from spinach leaf.
- F. Renosto, H. C. Patel, R. L. Martin, C. Thomassian, G. Zimmerman, I. H. Segel
- Biology, ChemistryArchives of biochemistry and biophysics
- 1 December 1993
Initial velocity measurements, product inhibition Studies, dead end inhibition studies, and kcat/Km comparisons support a random AB-ordered PQ kinetic mechanism in which MgATP and SO4(2-) bind in a highly synergistic manner.