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Structure-based Design of Prefusion-stabilized SARS-CoV-2 Spikes
Over 100 structure-guided spike variants based upon a previously determined cryo-EM structure of the prefusion SARS-CoV-2 spike are designed and expressed, with the best variant, HexaPro, having six beneficial proline substitutions leading to ∼10-fold higher expression than its parental construct.
Human cohesin compacts DNA by loop extrusion
It is shown that in the presence of the NIPBLMAU2 protein complex, the human cohesin complex can function as a molecular motor that extrudes DNA loops with high speed in vitro and compacts DNA in an ATP-dependent manner.
Structure-based design of prefusion-stabilized SARS-CoV-2 spikes
High-yield production of a stabilized prefusion spike protein will accelerate the development of vaccines and serological diagnostics for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).
Kinetic basis for DNA target specificity of CRISPR-Cas12a
- Isabel Strohkendl, Fatema A. Saifuddin, James R. Rybarski, I. Finkelstein, R. Russell
- Biology, ChemistrybioRxiv
- 26 June 2018
Class II CRISPR-Cas nucleases are programmable via a single guide RNA, enabling genome editing applications in nearly all organisms. However, DNA cleavage at off-target sites that resemble the target…
Prevalent, protective, and convergent IgG recognition of SARS-CoV-2 non-RBD spike epitopes
Plasma immunoglobulin G responses to the SARS-CoV-2 spike contain shared, or “public,” antibodies against a site that is recurrently mutated in variants of concern, and these data show that “ public” NTD-directed and other non-RBD plasma antibodies are prevalent and have implications for Sars-Cov-2 protection and antibody escape.
Single-Molecule Imaging Reveals How Mre11-Rad50-Nbs1 Initiates DNA Break Repair.
Frequency-frequency correlation functions and apodization in two-dimensional infrared vibrational echo spectroscopy: a new approach.
- K. Kwak, Sungnam Park, I. Finkelstein, M. Fayer
- PhysicsThe Journal of chemical physics
- 26 September 2007
A new observable is presented that greatly simplifies the extraction of the FFCF from experimental data that is the inverse of the center line slope (CLS) of the 2D spectrum and is essentially unaffected by Fourier filtering methods (apodization).
Single-molecule imaging reveals mechanisms of protein disruption by a DNA translocase
It is concluded that RecBCD overwhelms obstacles through direct transduction of chemomechanical force with no need for specific protein–protein interactions, and that proteins can be removed from DNA through active disruption mechanisms that act on a transition state intermediate as they are pushed from one nonspecific site to the next.
Assembly and Translocation of a CRISPR-Cas Primed Acquisition Complex
Single-molecule imaging of DNA curtains reveals mechanisms of KOPS sequence targeting by the DNA translocase FtsK
- Ja Yil Lee, I. Finkelstein, Estelle Crozat, D. Sherratt, E. Greene
- BiologyProceedings of the National Academy of Sciences
- 9 April 2012
The data support a model in which FtsK locates KOPS through random collisions, preferentially engages KOPS in the ADP-bound state, translocates in the direction dictated by the polar orientation of KOPS, and is incapable of recognizing KOPS while translocating along DNA.