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Flow cytometric determination of genome size in Pinus
Abstract The nuclear 2C DNA content of seven Pinus species and Pseudotsuga menziesii was determined by flow cytometric analysis. Conditions for nuclear isolation were adapted to optimise results forExpand
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Disruption of virus movement confers broad-spectrum resistance against systemic infection by plant viruses with a triple gene block.
White clover mosaic virus strain O (WClMV-O), species of the Potexvirus genus, contains a set of three partially overlapping genes (the triple gene block) that encodes nonvirion proteins of 26 kDa,Expand
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Detection of protoplast-derived DNA tetraploid Lisianthus (Eustoma grandiflorum) plants by leaf and flower characteristics and by flow cytometry
Plants of lisianthus (Eustoma grandiflorum (Griesbach)Schinners=Lisianthus russellianus Hook.) were regenerated from protoplasts and grown in pots until flowering. Vegetative and floralExpand
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Annexin-V and TUNEL use in monitoring the progression of apoptosis in plants.
An early indicator of apoptosis in mammalian cells is the loss of the phospholipid membrane asymmetry of the cell. This results in exposure of phosphatidylserine on the outer surface of the plasmaExpand
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Karyotypes, chromosome bands and genome size variation in New Zealand endemic gymnosperms
Karyotype studies on 20 taxa of gymnosperms endemic to New Zealand show a wide diversity of chromosome number and form. Fluorochrome banding with DAPI and CMA reveals a depauperate pattern of bandsExpand
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Major changes in chromatin condensation suggest the presence of an apoptotic pathway in plant cells.
A large decrease in fluorescence intensity of propidium iodide (PI)-stained nuclei is observed during senescence of plant cells. The phenomenon reflects a decrease in accessibility of DNA to thisExpand
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Xyloglucan endotransglycosylase activity during fruit development and ripening of apple and kiwifruit
Xyloglucan endotransglycosylase (XET) activity was measured in apple (Malus domestica Borkh. cv. Braeburn) pericarp and kiwifruit (Actinidia deliciosa [A. Chev.] C. F. Liang et A. R. Ferguson var.Expand
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Calcium signalling in programmed cell death in plants
Cell death is a major symptom of calcium deficiency in plants. This is likely to result from a breakdown in critical functions such as selective membrane permeability, and dysfunction of cellularExpand
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Protoplasts to plants of Gentianaceae. Regeneration of lisianthus (Eustoma grandiflorum) is affected by calcium ion preconditioning, osmolality and pH of the culture media
A reliable method has been developed for regeneration of whole plants from isolated protoplasts of five cultivars of lisianthus,Eustoma grandiflorum (Griseb.) Schinners (Gentianaceae). ProtoplastsExpand
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