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Effect of molecular crowding on self‐association of phosphorylase kinase and its interaction with phosphorylase b and glycogen
Self‐association of phosphorylase kinase (PhK) and its interaction with glycogen (M=5500 kDa) and phosphorylase b (Phb) has been studied using analytical ultracentrifugation and turbidimetry underExpand
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Site-selective agonist binding to the nicotinic acetylcholine receptor from Torpedo californica.
Fluorescent energy transfer measurements of dansyl-C6-choline binding to the nicotinic acetylcholine receptor (AChR) from Torpedo californica were used to determine binding characteristics of theExpand
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Effect of flavins on the rate of proteolytic digestion of muscle glycogen phosphorylase b.
The kinetics of tryptic proteolysis of rabbit skeletal muscle phosphorylase b has been registered by the diminishing of protein fluorescence intensity at lambda = 335 nm (excitation at 290 nm) or byExpand
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A tentative mechanism of the ternary complex formation between phosphorylase kinase, glycogen phosphorylase b and glycogen
The kinetics of rabbit skeletal muscle phosphorylase kinase interaction with glycogen has been studied. At pH 6.8 the binding of phosphorylase kinase to glycogen proceeds only in the presence ofExpand
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Site specificity of agonist-induced opening and desensitization of the Torpedo californica nicotinic acetylcholine receptor.
Agonist-binding kinetics to the nicotinic acetylcholine receptor (AChR) from Torpedo californica were measured using sequential-mixing stopped-flow fluorescence methods to determine the contributionExpand
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Mechanisms of interactions of the nucleotide cofactor with the RepA protein of plasmid RSF1010. Binding dynamics studied using the fluorescence stopped-flow method.
The dynamics of the nucleotide binding to a single, noninteracting nucleotide-binding site of the hexameric helicase RepA protein of plasmid RSF1010 has been examined, using the fluorescenceExpand
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The Regulatory α Subunit of Phosphorylase Kinase May Directly Participate in the Binding of Glycogen Phosphorylase
The yeast two-hybrid screen has been used to identify potential regions of interaction of the largest regulatory subunit, α, of phosphorylase kinase (PhK) with two fragments of its protein substrate,Expand
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Kinetics of the interaction of rabbit skeletal muscle phosphorylase kinase with glycogen.
The kinetics of the interaction of rabbit skeletal muscle phosphorylase kinase with glycogen was studied by the turbidimetric method at pH 6.8 and 8.2. Binding of phosphorylase kinase by glycogenExpand
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Dynamics of the ssDNA recognition by the RepA hexameric helicase of plasmid RSF1010: analyses using fluorescence stopped-flow intensity and anisotropy methods.
The kinetic mechanism of the single-stranded DNA (ssDNA) recognition by the RepA hexameric replicative helicase of the plasmid RSF1010 and the nature of formed intermediates, in the presence of theExpand
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Interaction of phosphorylase kinase from rabbit skeletal muscle with flavin adenine dinucleotide
The interaction of flavin adenine dinucleotide (FAD) with rabbit skeletal muscle phosphorylase kinase has been studied. Direct evidence of binding of phosphorylase kinase with FAD has been obtainedExpand
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